Effects of sulfur amino acids, L-methionine, L-cystine and L-cysteine on lipoprotein lipase and hormone-sensitive lipase in differentiated mouse 3T3-L1 adipocytes
Effects of sulfur amino acids, L-methionine, L-cystine and L-cysteine on lipoprotein lipase and hormone-sensitive lipase in differentiated mouse 3T3-L1 adipocytes
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DOI：
10.1007/s10616-010-9282-0
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发表时间：
2010-06-01
期刊：
影响因子：
2.2
通讯作者：
Yagasaki, Kazumi
中科院分区：
文献类型：
--
作者：
Kawasaki, Masashi;Miura, Yutaka;Yagasaki, Kazumi
皮下植入AH 109 A肝癌细胞的大鼠在肝癌生长过程中表现出高脂血症和高浓度的血清甘油三酯和非酯化脂肪酸，抑制脂蛋白脂肪酶（LPL），并升高对脂肪酸敏感的脂肪酶（HSL）的活性。在日粮中添加含硫氨基酸如L-蛋氨酸（Met）和L-胱氨酸（Cys）可通过恢复LPL和HSL活性来改善高脂血症。在本研究中，我们试图使用已知分化为脂肪细胞的3 T3-L1细胞来检查含硫氨基酸对LPL的活性和mRNA水平以及HSL的活性的影响。在不存在或存在肿瘤坏死因子-α（TNF-α）的情况下，将脂肪细胞与各种浓度的Met、Cys或L-半胱氨酸（CysH）孵育。LPL活性被TNF-α抑制。在不存在TNF-α的情况下，Met、Cys和CysH不改变LPL活性。在TNF-α存在下，Met和Cys显著增加LPL活性，并且Met还增加LPL mRNA水平。HSL活性也受到TNF-α的抑制。在TNF-α不存在的情况下，Met增强HSL活性。在TNF-α存在下，Met、Cys和CysH抑制HSL活性。含硫氨基酸如Met、Cys和CysH影响3 T3-L1脂肪细胞LPL活性、mRNA水平和HSL活性。含硫氨基酸的这些作用中的一些在LPL和HSL之间、在TNF-α的存在和不存在之间以及在3 T3-L1脂肪细胞和来自大鼠的脂肪组织之间是不同的。
Rats subcutaneously implanted with AH109A hepatoma cells show hyperlipidemia with high concentrations of serum triglyceride and nonesterified fatty acid, suppression of lipoprotein lipase (LPL), and elevation of hormone-sensitive lipase (HSL) activities during the growth of the hepatoma. Supplementation of the diet with sulfur amino acids such as L-methionine (Met) and L-cystine (Cys) improved hyperlipidemia by restoring LPL and HSL activities. In the present study, we have attempted to examine the effects of sulfur amino acids on the activity and mRNA level of LPL and the activity of HSL using 3T3-L1 cells, which are known to differentiate to adipocytes. The adipocytes were incubated with various concentrations of Met, Cys or L-cysteine (CysH) in the absence or presence of tumor necrosis factor-alpha (TNF-alpha). LPL activity was suppressed by TNF-alpha. In the absence of TNF-alpha, Met, Cys and CysH did not change the LPL activity. In the presence of TNF-alpha, Met and Cys significantly increased the LPL activity, and Met also enhanced the LPL mRNA level. HSL activity was also suppressed by TNF-alpha. In the absence of TNF-alpha, Met enhanced the HSL activity. In the presence of TNF-alpha, Met, Cys and CysH suppressed the HSL activity. Sulfur amino acids such as Met, Cys and CysH affected the LPL activity, mRNA level, and HSL activity in 3T3-L1 adipocytes. Some of these effects of sulfur amino acids were different between LPL and HSL, between the absence and the presence of TNF-alpha, and between 3T3-L1 adipocytes and the adipose tissue from rats.