Overexpression of ShDHN, a dehydrin gene from Solanum habrochaites enhances tolerance to multiple abiotic stresses in tomato
Overexpression of ShDHN, a dehydrin gene from Solanum habrochaites enhances tolerance to multiple abiotic stresses in tomato
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ShDHN（来自 Solanum habrochaites 的脱水蛋白基因）的过度表达可增强番茄对多种非生物胁迫的耐受性
DOI：
10.1016/j.plantsci.2014.12.006
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发表时间：
2015-02-01
期刊：
影响因子：
5.2
通讯作者：
Ye, Zhibiao
中科院分区：
文献类型：
--
作者：
Liu, Hui;Yu, Chuying;Ye, Zhibiao
脱水蛋白（DHN）在植物适应非生物胁迫中发挥重要作用。本研究从番茄野生种Solanum habrochaites中克隆了一个冷诱导的SK 3型DHN基因（ShDHN），并对其在非生物胁迫耐受中的功能进行了研究。ShDHN在根、叶、茎、花和果实中组成型表达。ShDHN在低温胁迫下表达持续上调，在耐冷的S. habrochaites比敏感S.番茄此外，ShDHN的表达还受到干旱、盐、渗透胁迫和外源信号分子的调控。在栽培番茄中过量表达ShDHN可以提高番茄对寒冷和干旱胁迫的耐受性，并改善盐和渗透胁迫下的幼苗生长。与野生型相比，转基因植株积累了更多的脯氨酸，保持了较高的超氧化物歧化酶和过氧化氢酶的酶活性，并在寒冷和干旱胁迫下遭受较少的膜损伤。此外，转基因植株在低温胁迫下积累的H2 O2和O-2（-）水平较低，在脱水条件下具有较高的相对含水量和较低的失水速率。此外，ShDHN在番茄中的过量表达导致了参与ROS清除和JA信号通路的几个基因的表达上调或下调，包括SOD 1，GST，POD，LOX，PR 1和PR 2。综上所述，这些结果表明，ShDHN在提高植物对非生物胁迫的适应性方面具有多效性效应，并且它在番茄耐逆性的遗传改良中具有潜在的用途。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Dehydrins (DHNs) play important roles in plant adaptation to abiotic stress. In this study, a cold-induced SK3-type DHN gene (ShDHN) isolated from wild tomato species Solanum habrochaites was characterized for its function in abiotic stress tolerance. ShDHN was constitutively expressed in root, leaf, stem, flower and fruit. ShDHN was continuously up-regulated during cold stress and showed higher expression level in the cold-tolerant S. habrochaites than in the susceptible S. lycopersicum. Moreover, ShDHN expression was also regulated by drought, salt, osmotic stress, and exogenous signaling molecules. Overexpression of ShDHN in cultivated tomato increased tolerance to cold and drought stresses and improved seedling growth under salt and osmotic stresses. Compared with the wild-type, the transgenic plants accumulated more proline, maintained higher enzymatic activities of superoxide dismutase and catalase, and suffered less membrane damage under cold and drought stresses. Moreover, the transgenic plants accumulated lower levels of H2O2 and O-2(-) under cold stress, and had higher relative water contents and lower water loss rates under dehydration conditions. Furthermore, overexpression of ShDHN in tomato led to the up- or down-regulated expression of several genes involved in ROS scavenging and JA signaling pathway, including SOD1, GST, POD, LOX, PR1 and PR2. Taken together, these results indicate that ShDHN has pleiotropic effects on improving plant adaptation to abiotic stresses and that it possesses potential usefulness in genetic improvement of stress tolerance in tomato. (C) 2014 Elsevier Ireland Ltd. All rights reserved.