Neural crest-derived cells in nasal conchae of adult mice contribute to bone regeneration
Neural crest-derived cells in nasal conchae of adult mice contribute to bone regeneration
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成年小鼠鼻甲中的神经嵴衍生细胞有助于骨再生
DOI：
10.1016/j.bbrc.2021.03.079
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发表时间：
2021
影响因子：
3.1
通讯作者：
Kamijo Ryutaro
中科院分区：
文献类型：
--
作者：
Yoshida Hiroshi;Suzawa Tetsuo;Shibata Yo;Takahashi Masahiro;Kawai Ryota;Takami Masamichi;Maki Koutaro;Kamijo Ryutaro
神经脊源性细胞(NCDCs)是一类不局限于胚胎组织的成体干细胞，由于其易于分离、自我更新和多能性，是一种有吸引力的组织再生治疗候选细胞。尽管成年NCDCs可以在体外进行成骨分化，但它们是否在体内诱导成骨尚不清楚。此前，我们的研究小组报告了大量NCDC散布在成年小鼠的鼻甲组织中的发现。本研究从成年P0-Cre/CAG-CAT-EGFP双转基因小鼠的鼻甲中收集标记了增强型绿色荧光蛋白(EGFP)的NCDCs，并在无血清条件下进行培养以增加其数量。取NCDCs，悬浮于I型胶原凝胶中，以胶原海绵为支架进行细胞悬浮。将含有NCDCs的Atelocollagen支架放置在小鼠颅骨缺损模型的骨缺损上。在接下来的12周里，Micro-CT和组织学分析结果显示，与只使用支架的小鼠相比，使用含有NCDCs的支架的小鼠有稍微更大的骨形成。此外，拉曼光谱显示了接受NCDCs支架的小鼠骨骼的光谱特性，这些支架与天然头盖骨相似。骨再生不仅对增加骨量很重要，而且对化学性质也很重要。这些结果首次证明了无生物分子的成人鼻甲来源NCDCs用于骨再生的有效性，包括再生骨组织的化学性质。
Neural crest-derived cells (NCDCs), a class of adult stem cells not restricted to embryonic tissues, are attractive tissue regenerative therapy candidates because of their ease of isolation, self-renewing properties, and multipotency. Although adult NCDCs can undergo osteogenic differentiationin vitro, whether they induce bone formationin vivoremains unclear. Previously, our group reported findings showing high amounts of NCDCs scattered throughout nasal concha tissues of adult mice. In the present study, NCDCs in nasal conchae labeled with enhanced green fluorescent protein (EGFP) were collected from adultP0-Cre/CAG-CAT-EGFPdouble transgenic mice, then cultured in serum-free medium to increase the number. Subsequently, NCDCs were harvested and suspended in type I atelocollagen gel, then an atelocollagen sponge was used as a scaffold for the cell suspension. Atelocollagen scaffolds with NCDCs were placed on bone defects created in a mouse calvarial bone defect model. Over the ensuing 12 weeks, micro-CT and histological analysis findings showed that mice with scaffolds containing NCDCs had slightly greater bone formation as compared to those with a scaffold alone. Furthermore, Raman spectroscopy revealed spectral properties of bone in mice that received scaffolds with NCDCs similar to those of native calvarial bone. Bone regeneration is important not only for gaining bone mass but also chemical properties. These results are the first to show the validity of biomolecule-free adult nasal concha-derived NCDCs for bone regeneration, including the chemical properties of regenerated bone tissue.