Prion propagation in cultured cells.
Prion propagation in cultured cells.
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朊病毒在培养细胞中的繁殖。
DOI：
10.1093/bmb/66.1.87
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发表时间：
2003
影响因子：
6.7
通讯作者：
S. Lehmann
中科院分区：
文献类型：
--
作者：
J. Solassol;C. Crozet;S. Lehmann
细胞培养物代表了传染性海绵状脑病（TSE）的相关和有用的实验模型。它们能够在传代后促进PrP（Sc）的稳定和持续复制以及感染性。迄今为止，只有少数细胞培养模型允许朊病毒复制。其中，小鼠神经母细胞瘤细胞系（N2a）是最常用的。虽然它们对应于支持小鼠适应性羊瘙痒病菌株繁殖的同源模型，但最近的研究报告了对自然发生的疾病敏感的异源模型。感染的细胞培养模型提供了一些有价值的见解PrP（Sc）的生物发生的转换，亚细胞定位，病理生理后果和物种屏障的决定因素。他们还有助于筛选和研究可能的治疗化合物，并为研究TSE特异性诊断标志物制定新策略。
Cell cultures represent relevant and useful experimental models of transmissible spongiform encephalopathies (TSEs). They are able to promote, upon subpassaging, stable and persistent replication of PrP(Sc) as well as infectivity. To date, only a few cell culture models permissive to prion replication are available. Among them, mouse neuroblastoma cell lines (N2a) are most commonly used. While they correspond to homologous models supporting propagation of mouse-adapted scrapie strains, recent studies have reported heterologous models sensitive to natural occurring disease. Infected cell culture models have provided some valuable insights into the biogenesis of PrP(Sc) in terms of conversion, subcellular localisations, physiopathological consequences and species-barrier determinants. They have also contributed to the screening and the study of possible therapeutic compounds and to the development of new strategies for the investigation of TSE-specific diagnostic markers.