Genetic footprinting: a genomic strategy for determining a gene's function given its sequence.
Genetic footprinting: a genomic strategy for determining a gene's function given its sequence.
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DOI：
10.1073/pnas.92.14.6479
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发表时间：
1995-07
影响因子：
11.1
通讯作者：
V. Smith;D. Botstein;Patrick O. Brown
中科院分区：
文献类型：
--
作者：
V. Smith;D. Botstein;Patrick O. Brown
本报告描述了一种确定微生物中测序基因功能的有效策略。大量的细胞受到插入突变的影响。然后将诱变的种群分成有代表性的样本，每个样本都要进行不同的选择。选择后从每个样本群体中制备DNA。然后使用聚合酶链反应来回顾性地确定插入到特定序列中是否影响任何选择的结果。该方法是高效的，因为插入诱变和每次选择只需要执行一次，就可以研究数千个基因的功能，而不是对每个基因进行一次。我们使用模式生物酿酒酵母测试了这种“遗传足迹”策略。
This report describes an efficient strategy for determining the functions of sequenced genes in microorganisms. A large population of cells is subjected to insertional mutagenesis. The mutagenized population is then divided into representative samples, each of which is subjected to a different selection. DNA is prepared from each sample population after the selection. The polymerase chain reaction is then used to determine retrospectively whether insertions into a particular sequence affected the outcome of any selection. The method is efficient because the insertional mutagenesis and each selection need only to be performed once to enable the functions of thousands of genes to be investigated, rather than once for each gene. We tested this "genetic footprinting" strategy using the model organism Saccharomyces cerevisiae.