Evaluation of head and neck squamous cell carcinoma invasiveness by the electrical resistance breakdown assay
Evaluation of head and neck squamous cell carcinoma invasiveness by the electrical resistance breakdown assay
复制标题
电阻击穿实验评价头颈鳞状细胞癌的侵袭性
DOI：
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发表时间：
2005
影响因子：
4
通讯作者：
J. Werner
中科院分区：
文献类型：
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作者：
R. Mandic;T. Ludwig;H. Oberleithner;J. Werner
肿瘤细胞侵入周围组织是癌症的标志。头颈部鳞状细胞癌（HNSCC）的特点是早期主要淋巴转移扩散。本研究的目的是评估使用电阻击穿测定确定HNSCC肿瘤细胞侵袭力。该试验利用上皮MDCK-C7单层的高跨上皮电阻（TEER）作为单层完整性和渗透性的敏感指标。MDCK-C7细胞在微滤膜杯中生长至汇合。将3×106个来自HNSCC肿瘤的细胞系UM-SCC-3、UM-SCC-27、UMB-SCC-745、UMB-SCC-864、UMB-SCC-969和UT-SCC-26 A的癌细胞接种在该上皮测试屏障上。A7-黑色素瘤细胞用作阳性对照，而MDCK-C7细胞用作阴性对照，并以与测试的肿瘤细胞相同的数量应用。在接下来的几天内测量TEER并与对照值进行比较。在前72小时内，在UMB-SCC-745、UMB-SCC-969和UT-SCC-26 A细胞系中观察到TEER显著降低，而在UMB-SCC-3、UMB-SCC-27和UMB-SCC-864细胞系中未观察到TEER显著降低。通常发现HNSCC细胞系在抗性破坏测定中与其它肿瘤细胞如A7-黑素瘤细胞相比侵袭性较小，然而，电阻击穿分析似乎能够证明不同HNSCC细胞系之间侵袭性的差异，因此可能用作区分高侵袭性和低侵袭性肿瘤的通用工具，具有作为诊断和预后的潜在应用。临床研究中的标志物。
Invasion of tumor cells into the surrounding tissue is a hallmark of cancer. Squamous cell carcinomas of the head and neck region (HNSCC) are characterized by their early primarily lymphatic metastatic spread. The aim of the present study was to evaluate the use of the electrical resistance breakdown assay for determining HNSCC tumor cell invasiveness. The assay utilizes the high transepithelial electrical resistance (TEER) of an epithelial MDCK-C7 monolayer as a sensitive indicator of monolayer integrity and permeability. MDCK-C7 cells were grown to confluence in microfilter membrane cups. 3×106 cancer cells of cell lines UM-SCC-3, UM-SCC-27, UMB-SCC-745, UMB-SCC-864, UMB-SCC-969 and UT-SCC-26A derived from HNSCC tumors, were seeded on top of this epithelial test barrier. A7-melanoma cells served as a positive control whereas MDCK-C7 cells were used as a negative control and were applied in the same number as the tested tumor cells. TEER was measured over the following days and compared to control values. A significant reduction in TEER was observed in the UMB-SCC-745, UMB-SCC-969 and UT-SCC-26A cell lines within the first 72 h, whereas no significant reduction in TEER was seen in the UM-SCC-3, UM-SCC-27 and UMB-SCC-864 cell lines. HNSCC cell lines in general are found to be less invasive in the resistance breakdown assay compared to other tumor cells such as A7-melanoma cells, however, the electrical resistance breakdown assay appears capable of demonstrating differences in invasiveness between different HNSCC cell lines and therefore potentially could serve as a versatile tool in distinguishing high and low invasive tumors with a potential application as a diagnostic and prognostic marker in clinical investigations.