Bovine trophoblastic cell differentiation on collagen substrata: formation of binucleate cells expressing placental lactogen
Bovine trophoblastic cell differentiation on collagen substrata: formation of binucleate cells expressing placental lactogen
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DOI：
10.1007/s00441-001-0491-x
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发表时间：
2002-02-01
影响因子：
3.6
通讯作者：
Hashizume, K
中科院分区：
文献类型：
--
作者：
Nakano, H;Shimada, A;Hashizume, K
反刍动物滋养外胚层分化的标志是细胞滋养层中出现双核细胞。双核细胞由细胞滋养层的无细胞分裂产生，并进行核内复制。它们分泌激素，如胎盘催乳素，并表现出迁移行为，将其激素转移到母体循环中。本研究以体外受精的囊胚为材料，建立了牛滋养层细胞系（BT-1），并在胶原凝胶上诱导其分化为双核细胞。BT-1具有细胞滋养层上皮特征，表达细胞角蛋白、E-cadherin和干扰素-tau。它自发形成多细胞球形囊泡漂浮在培养基中。我们在I型胶原基质上培养这些囊泡。大多数囊泡附着在胶原基质上，并表现出细胞生长和增殖。我们发现，10天后，胶原凝胶上的细胞集落中出现双核细胞簇，但胶原膜上没有。这些双核细胞具有体内特征，包括核DNA含量增加和胎盘催乳素表达。BT-1是研究反刍动物滋养细胞分化的一个有用的模型。
The differentiation of trophectoderm in ruminants is marked by the appearance of binucleate cells in cytotrophoblasts. Binucleate cells are produced by the acytokinesis of cytotrophoblasts and undergo endoreduplication. They secrete hormones such as placental lactogen, and exhibit migratory behavior to transfer their hormones into maternal circulations. In this study, we showed that a bovine trophoblastic cell line (BT-1) established from in vitro fertilized blastocysts differentiated into binucleate cells on Collagen gel. BT-1 had cytotrophoblastic epithelial characteristics in that it expressed cytokeratin, E-cadherin and interferon-tau. It spontaneously formed multicellular spherical vesicles floating in the medium. We cultured these vesicles on type I collagen substrata. Most vesicles attached to the Collagen substrata, and exhibited cell outgrowth and proliferation. We found that after more than 10 days, clusters of binucleate cells appeared in the cell colonies on the Collagen gel, but not on the Collagen film. These binucleate cells have features characteristic of those in vivo, including an increased nuclear DNA content and the expression of placental lactogen. BT-1 is a useful model with which to study trophoblast differentiation in ruminants.