Antivirus antibody-dependent cell-mediated cytotoxicity during murine cytomegalovirus infection
Antivirus antibody-dependent cell-mediated cytotoxicity during murine cytomegalovirus infection
复制标题
鼠巨细胞病毒感染期间抗病毒抗体依赖性细胞介导的细胞毒性
作者：
J. Manischewitz;G. Quinnan,
研究人员对感染鼠巨细胞病毒的 BALB/c 小鼠进行了研究，以确定抗体依赖性细胞介导的细胞毒性是否有助于这种感染的免疫控制。来自未感染小鼠的抗体依赖性杀伤细胞被用作效应细胞来测定感染小鼠血清中的抗体。发现二次免疫血清含有巨细胞病毒特异性抗体和自身反应性抗体。初次感染后，仅发现巨细胞病毒特异性抗体。这些是在感染后 8 至 10 天内通过抗体依赖性细胞介导的细胞毒性检测到的，但通常要到第 21 天才通过中和抗体测定检测。抗体依赖性细胞介导的细胞毒性比中和法的抗体滴度高约 10 倍。结果表明，针对巨细胞病毒感染的细胞免疫包括抗体依赖性细胞介导的细胞毒性反应，该反应可能非常有效，并且可能对感染的急性期和后期阶段的控制做出重大贡献。
BALB/c mice infected with murine cytomegalovirus were studied to determine whether antibody-dependent cell-mediated cytotoxicity contributes to the immune control of this infection. Antibody-dependent killer cells from uninfected mice were used as effector cells to assay for antibody in sera of infected mice. Secondary immune sera were found to contain both cytomegalovirus-specific and autoreactive antibodies. After primary infection only cytomegalovirus-specific antibodies were found. These were detected by antibody-dependent cell-mediated cytotoxicity within 8 to 10 days after onset of infection, but usually not until day 21, by a neutralizing antibody assay. Antibody titers were about 10-fold higher by antibody-dependent cell-mediated cytotoxicity than by neutralization. The results indicate that cellular immunity to cytomegalovirus infection includes an antibody-dependent cell-mediated cytotoxicity response which is likely to be highly efficient and may contribute significantly to control of both acute and later stages of infection.