Tubulin bistability and polymorphic dynamics of microtubules.
Tubulin bistability and polymorphic dynamics of microtubules.
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微管蛋白双稳定性和微管的多态动力学。
DOI：
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发表时间：
2010
影响因子：
8.6
通讯作者：
I. Kulić
中科院分区：
文献类型：
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作者：
H. Mohrbach;A. Johner;I. Kulić
基于假设gdp -微管蛋白二聚体是一种构象双稳定分子-在离散曲线和直线状态之间快速波动-我们开发了微管晶格的多态动力学模型。我们表明，gdp -微管蛋白双稳定性一致地解释了不寻常的动态波动，嫁接紫杉醇稳定微管的明显长度-刚度关系，以及微管的弯曲-螺旋外观。当被一端夹住时，微管会经历一种不寻常的零能量运动——其效果让人想起一个有限的旋转铰链。我们得出结论，微管存在于高度合作的能量简并螺旋状态，并讨论了体内可能的影响。
Based on the hypothesis that the GDP-tubulin dimer is a conformationally bistable molecule-rapidly fluctuating between a discrete curved and a straight state-we develop a model for polymorphic dynamics of the microtubule lattice. We show that GDP-tubulin bistability consistently explains unusual dynamic fluctuations, the apparent length-stiffness relation of grafted taxol-stabilized microtubules, and the curved-helical appearance of microtubules in general. When clamped by one end the microtubules undergo an unusual zero energy motion-in its effect reminiscent of a limited rotational hinge. We conclude that microtubules exist in highly cooperative energy-degenerate helical states and discuss possible implications in vivo.