Expression of apoptotic factors in vaginal tissues from women with urogenital prolapse.
Expression of apoptotic factors in vaginal tissues from women with urogenital prolapse.
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DOI：
10.1002/nau.21127
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发表时间：
2011-11
影响因子：
2
通讯作者：
Chen B
中科院分区：
文献类型：
--
作者：
Wen Y;Ho JY;Polan ML;Chen B
盆腔组织中细胞凋亡活性的增加可能有助于盆底疾病的发展。我们评估了盆腔器官脱垂（POP）妇女阴道组织中凋亡因子（Bcl-2家族）的表达，以及这些因子与脱垂严重程度的相关性。采用实时荧光定量PCR和Western blot检测阴道组织中抗凋亡因子和促凋亡因子的mRNA和蛋白表达。脱垂的严重程度采用POP-Q标准进行分期。Bcl-2家族因子的差异表达是在蛋白质而不是基因表达中观察到的。在分泌期，对照组的抗凋亡蛋白Bcl-2、Bcl-xl和促凋亡蛋白Bax表达均高于病例组（P < 0.05）。Bcl-2/Bax和Bcl-2/Bad的比率，决定细胞对诱导凋亡的敏感性，在对照组中高于病例组。较高的比率表明细胞对凋亡的敏感性降低。重度脱垂组Bax和Bad蛋白表达高于轻度脱垂组（P < 0.05）。Bad、Bax表达增加，Bcl-2/Bax、Bcl-2/Bad比值降低，提示POP患者阴道组织中细胞凋亡活性增加或对诱导细胞凋亡的敏感性增加。
Increased apoptotic activity in pelvic tissues may contribute to development of pelvic floor disorders. We evaluated expression of apoptotic factors (Bcl-2 family) in vaginal tissues from women with pelvic organ prolapse (POP) and how these factors correlate with severity of prolapse. mRNA and protein expression of anti-apoptotic and pro-apoptotic factors in vaginal tissues from subjects and controls were determined by real-time PCR and Western blot. Severity of prolapse was staged using POP-Q criteria. Differential expression of Bcl-2 family factors was observed in protein rather than in gene expression. During the secretory phase, the anti-apoptotic (Bcl-2, Bcl-xl) and pro-apoptotic protein (Bax) were upregulated in controls compared to cases (P < 0.05). The ratios of Bcl-2/Bax and Bcl-2/Bad, which determine cellular sensitivity to induction of apoptosis, were higher in controls versus cases. Higher ratios indicate reduced cellular sensitivity to apoptosis. Protein expression of Bax and Bad was higher in women with severe compared to mild prolapse (P < 0.05). Increased expression of Bad, Bax, and decreased ratios of Bcl-2/Bax, Bcl-2/Bad suggest increased apoptotic activity or sensitivity to induction of apoptosis in vaginal tissues of women with POP.