Environment of ribothymidine in transfer ribonucleic acid studied by means of nuclear Overhauser effect.
Environment of ribothymidine in transfer ribonucleic acid studied by means of nuclear Overhauser effect.
复制标题
利用核奥弗豪瑟效应研究核糖核酸中核糖核酸的环境。
DOI：
10.1021/bi00511a010
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Redfield,AG
中科院分区：
文献类型：
--
作者：
Tropp,J;Redfield,AG
James Tropp和Alfred G. Redfield* 摘要：在大肠杆菌tRNAVal、E. coli tRNA™ 61和酵母tRNAPhe。还观察到T甲基的特异性倒数NOE。使用差光谱允许甚至在光谱的芳族区域中观察NOE;所有实验都在H2O溶剂中进行。从NOE到T甲基确定了NOE耦合共振对的交叉弛豫速率;将测量速率与基于tRNA Phecrystal结构的理论速率进行比较，在所有这些tRNA中，完全分辨的55 Ni质子共振在10.6 ppm附近，T54 C6和55 C6质子共振在7.3 ppm附近。这55个样本经55 Nt和20 Nt之间的NOE确认，
James Tropp and Alfred G. Redfield* abstract: Specific nuclear Overhauser effects (NOE’s) were observed from the thymine methyl proton resonance to several proton resonances in the range 6.8-11 ppm from DSS, in Escherichia coli tRNAVal, E. coli tRNA™ 61, and yeast tRNAPhe. Specific reciprocal NOE’s to the T methyl were also observed. The use of difference spectroscopy allowed observation of NOE’s even in the aromatic region of the spectrum; all experiments were done in H20 solvent. Cross relaxation rates for pairs of NOE-coupled resonances were determined from the NOE’s to the T methyl; comparison of measured rates with theoretical ratesbased on tRNAPhecrystal structure led to assignment of the fully resolved 55 N i proton resonance near 10.6 ppm and the T54 C6 and 55 C6 proton resonances near 7.3 ppm in all these tRNAs. The 55 as-signments were confirmed by NOE’s between the 55 Nt and