Immunocytochemical localization of carcinoembryonic antigen in benign and malignant colorectal tissues. Assessment of diagnostic value.
Immunocytochemical localization of carcinoembryonic antigen in benign and malignant colorectal tissues. Assessment of diagnostic value.
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良性和恶性结直肠组织中癌胚抗原的免疫细胞化学定位。
DOI：
10.1093/ajcp/75.3.283
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发表时间：
1981
影响因子：
3.5
通讯作者：
Gottlieb,LS
中科院分区：
文献类型：
--
作者：
O'Brien,MJ;Zamcheck,N;Burke,B;Kirkham,SE;Saravis,CA;Gottlieb,LS
对70例结肠癌、20例肝及淋巴结转移瘤、150例肠系膜淋巴结、40例癌旁粘膜、20例结肠切缘、10例正常结肠组织的石蜡切片和冰冻切片分别进行癌胚抗原（CEA）的免疫过氧化物酶和免疫荧光染色，和结直肠息肉（64），以评估其潜在的诊断价值。在本研究的基础上，对大肠组织中癌胚抗原的免疫细胞化学定位进行了研究，得出以下结论。(1)CEA定位于表面上皮细胞的糖萼是结肠中的正常发现，并且在正常结肠和远离和邻近浸润性癌的粘膜中相似。(2)虽然强阳性细胞表面和管腔内染色经常与肿瘤性息肉中癌的存在相关，但其本身并不是可靠的诊断标准。(3)未能在腺体形成癌中显示CEA使得结直肠癌的诊断不太可能。(4)低分化的大肠癌通常含有较少的CEA，但偶尔可以强烈染色的细胞质。(5)虽然结直肠癌淋巴结微转移可以通过免疫过氧化物酶CEA染色来证实，但由有能力的病理学家对苏木精和伊红染色切片进行筛查似乎足以检测到这些微转移。
Immunoperoxidase and immunofluorescence staining for carcinoembryonic antigen (CEA) was performed on paraffin and frozen sections, respectively, of colonic carcinomas (70), liver and lymph node metastases (20), mesenteric nodes (150), mucosa adjacent to carcinoma (40), colonic resection margins (20), normal colon (ten), and colorectal polyps (64) in order to assess its potential diagnostic value. On the basis of this study of the immunocytochemical localization of CEA in colorectal tissues, conclusions were as follows. (1) Localization of CEA to glycocalyx of surface epithelial cells is a normal finding in the colon and is similar in normal colon and mucosa distant and adjacent to infiltrating carcinoma. (2) Although strongly positive cell surface and intraluminal staining frequently correlates with the presence of carcinoma in neoplastic polyps, it is not by itself a reliable diagnostic criterion. (3) Failure to demonstrate CEA in a gland-forming carcinoma makes a diagnosis of colorectal carcinoma unlikely. (4) Poorly differentiated colorectal carcinoma usually contains much less demonstrable surface CEA, but may occasionally stain cytoplasm strongly. (5) Although lymph node micrometastases from colorectal carcinoma are readily demonstrated by immunoperoxidase staining for CEA, screening of hematoxylin and eosin-stained sections by a competent pathologist appears to be adequate for their detection.