Application of an ex ovo chicken chorioallantoic membrane model for two-photon excitation photodynamic therapy of age-related macular degeneration.
Application of an ex ovo chicken chorioallantoic membrane model for two-photon excitation photodynamic therapy of age-related macular degeneration.
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DOI：
10.1117/1.1577117
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发表时间：
2003-07
影响因子：
3.5
通讯作者：
K. Samkoe;D. Cramb
中科院分区：
文献类型：
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作者：
K. Samkoe;D. Cramb
双光子激发光动力疗法（TPE-PDT）是一种治疗老年性黄斑变性（AMD）的临床方法。与传统的单光子激发PDT相比，TPE-PDT有可能提供一种更具体、更有利的治疗方案。利用8 ~ 9日龄高度血管化的鸡绒毛尿囊膜（CAM）来模拟湿型AMD血管的快速生长。使用CAM的卵外模型系统，消融研究成功地模拟了AMD中发现的渗漏血管。此外，研究了脂质体的分布和定位，以表征光敏药物的体内特性。光敏剂在血管上壁的定位似乎最大，这表明TPE-PDT的潜在强治疗部位。
Two-photon excitation photodynamic therapy (TPE-PDT) is being investigated as a clinical treatment for age-related macular degeneration (AMD). TPE-PDT has the potential to provide a more specific and therefore advantageous therapy regime than traditional one-photon excitation PDT. The highly vascularized 8 to 9-day-old chicken chorioallantoic membrane (CAM) is used to model the rapid growth of blood vessels in the wet form of AMD. Using an ex ovo model system for the CAM, ablation studies were successful in mimicking the leaky vessels found in AMD. In addition, the distribution and localization of liposomal Verteporfin were investigated in order to characterize the photosensitizing drug in vivo. Localization of the photosensitizer appears to be greatest on the upper vessel wall, which indicates a potentially strong treatment locale for TPE-PDT.