Differential expression of high molecular weight caldesmon in colorectal pericryptal fibroblasts and tumour stroma
Differential expression of high molecular weight caldesmon in colorectal pericryptal fibroblasts and tumour stroma
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DOI：
10.1136/jcp.52.10.785
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发表时间：
1999-10-01
影响因子：
3.4
通讯作者：
Enzan, H
中科院分区：
文献类型：
--
作者：
Nakayama, H;Miyazaki, E;Enzan, H
目的-为了调查的程度，平滑肌分化的周ryptal成纤维细胞。方法-表达高分子量钙调蛋白（h-CD）和平滑肌肌动蛋白进行了研究，在α浸润性结直肠腺癌及其周围的非肿瘤组织。结果-h-CD的单克隆抗体，这主要是阳性免疫染色在分化良好的平滑肌细胞，识别周ryptal成纤维细胞，平滑肌细胞，和周细胞，但不反应肌成纤维细胞。平滑肌肌动蛋白的抗体不仅承认pericryptal成纤维细胞，平滑肌细胞，周细胞，但也myofibroblasts. Conclusions-pericryptal成纤维细胞显示更大的平滑肌分化比myofibroblasts和有可能，他们是分化良好的平滑肌细胞; h-CD是一个很好的标记区分pericryptal成纤维细胞myofibroblasts。
Aim-To investigate an extent of smooth muscle differentiation of pericryptal fibroblasts.Methods-Expression of high molecular weight caldesmon (h-CD) and a smooth muscle actin was investigated in alpha invasive colorectal adenocarcinomas and their surrounding non-neoplastic tissues.Results-The monoclonal antibody to h-CD, which showed predominantly positive immunostaining in well differentiated smooth muscle cells, recognised pericryptal fibroblasts, smooth muscle cells, and pericytes, but was not reactive to myofibroblasts. Antibody to a smooth muscle actin recognised not only pericryptal fibroblasts, smooth muscle cells, and pericytes but also myofibroblasts.Conclusions-Pericryptsal fibroblasts show greater smooth muscle differentiation than myofibroblasts and there is a possibility that they are well differentiated smooth muscle cells; h-CD is an excellent marker to discriminate pericryptal fibro blasts from myofibroblasts.