Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice
Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice
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DOI：
10.1073/pnas.0604882103
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发表时间：
2006-08-29
影响因子：
11.1
通讯作者：
Xiong, Lizhong
中科院分区：
文献类型：
--
作者：
Hu, Honghong;Dai, Mingqiu;Xiong, Lizhong
干旱和盐度是作物生产的主要非生物压力。在这里，我们表明，在严重的干旱应力条件下，在繁殖阶段，在野外，应力响应基因SNAC1（应力反应性NAC 1）显着增强了转基因水稻中的干旱性（比对照高22-34％）。没有表型变化或产量罚款。在营养阶段，转基因大米还显示出明显改善的耐干旱和耐盐性。与WT相比，转基因大米对脱落酸更敏感，并且通过关闭更多的气孔毛孔而更慢地损失水，但是光合作用速率没有显着差异。 SNAC1主要通过干旱诱导后卫细胞，并用反式激活活性编码A NAM，ATAF和CUC（NAC）转录因子。 DNA芯片分析表明，在过表达SNAC1的水稻植物中，大量与应力相关的基因被上调。我们的数据表明，SNAC1在改善大米的干旱和盐度耐受性方面具有有希望的实用性。
Drought and salinity are major abiotic stresses to crop production. Here, we show that overexpression of stress responsive gene SNAC1 (STRESS-RESPONSIVE NAC 1) significantly enhances drought resistance in transgenic rice (22-34% higher seed setting than control) in the field under severe drought stress conditions at the reproductive stage while showing no phenotypic changes or yield penalty. The transgenic rice also shows significantly improved drought resistance and salt tolerance at the vegetative stage. Compared with WT, the transgenic rice are more sensitive to abscisic acid and lose water more slowly by closing more stomatal pores, yet display no significant difference in the rate of photosynthesis. SNAC1 is induced predominantly in guard cells by drought and encodes a NAM, ATAF, and CUC (NAC) transcription factor with transactivation activity. DNA chip analysis revealed that a large number of stress-related genes were up-regulated in the SNAC1-overexpressing rice plants. Our data suggest that SNAC1 holds promising utility in improving drought and salinity tolerance in rice.