Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat
Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat
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DOI：
10.1038/nn974
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发表时间：
2002-12-01
影响因子：
25
通讯作者：
Yu, YJ
中科院分区：
文献类型：
--
作者：
Wu, P;Tarasenko, YI;Yu, YJ
从人类胚胎或成人中枢神经系统(CNS)分离的多能或多能干细胞可以提供新的神经元来改善神经疾病。然而，一个主要的障碍是，当移植到成人中枢神经系统的非神经源性区域时，大多数这样的细胞不能分化为神经元。在这里，我们报道了一种新的体外启动程序，该程序从移植到成年大鼠中枢神经系统的胎儿神经干细胞(HNSCs)产生几乎纯的神经元群体。此外，移植细胞通过以区域特有的方式获得胆碱能表型而分化。这项技术可能会推进基于干细胞的治疗，以取代神经损伤或神经退行性疾病中丢失的神经元。
Pluripotent or multipotent stem cells isolated from human embryos or adult central nervous system (CNS) may provide new neurons to ameliorate neural disorders. A major obstacle, however, is that the majority of such cells do not differentiate into neurons when grafted into non-neurogenic areas of the adult CNS. Here we report a new in vitro priming procedure that generates a nearly pure population of neurons from fetal human neural stem cells (hNSCs) transplanted into adult rat CNS. Furthermore, the grafted cells differentiated by acquiring a cholinergic phenotype in a region-specific manner. This technology may advance stem cell-based therapy to replace lost neurons in neural injury or neurodegenerative disorders.