Transcriptional regulation of the MHC class I HLA-A11 promoter by the zinc finger protein ZFX
Transcriptional regulation of the MHC class I HLA-A11 promoter by the zinc finger protein ZFX
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DOI：
10.1093/nar/24.10.1928
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发表时间：
1996-05-15
影响因子：
14.9
通讯作者：
Gazin, C
中科院分区：
文献类型：
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作者：
LHaridon, M;Paul, P;Gazin, C
人类MHC I类HLA-A11启动子的调控是由一系列复杂的调控元件控制的。其中一个对启动子转录活性至关重要的元件被用来筛选lambda gt11文库，并鉴定出编码锌指蛋白ZFX的cDNA。ZFX可以结合分别位于HLA-A11启动子-271 ~ -263和-242 ~ -234位置的序列AGGGCCCCA和AGGCCCCGA，通过其三个c端锌指具有相似的亲和力。ZFX（575）是ZFX的短异构体，在间质细胞系中激活HLA-A11启动子的转录。
Regulation of the human MHC class I HLA-A11 promoter is governed by a complex array of regulatory elements. One of these elements, shown here to be critical for the transcriptional activity of the promoter, was used to screen a lambda gt11 library and allowed the identification of a cDNA which coded for the zinc finger protein ZFX. ZFX was shown to bind the sequences AGGGCCCCA and AGGCCCCGA, located respectively at positions -271 to -263 and -242 to -234 of the HLA-A11 promoter, with similar affinities through its three C-terminal zinc fingers. ZFX(575), a short isoform of ZFX, activates transcription from the HLA-A11 promoter in a Leydig cell line.