Design of dual-emission chemosensors for ratiometric detection of ATP derivatives
Design of dual-emission chemosensors for ratiometric detection of ATP derivatives
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DOI：
10.1002/asia.200600137
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发表时间：
2006-10-01
影响因子：
4.1
通讯作者：
Hamachi, Itaru
中科院分区：
文献类型：
--
作者：
Ojida, Akio;Miyahara, Yoshifumi;Hamachi, Itaru
焦磷酸核苷（核苷PP）衍生物广泛存在于活细胞中，在各种生物事件中起着关键作用。我们报道了利用配位化学的新型核苷PPs荧光化学传感器。化学传感器包含两个zn - ii -二聚胺单元，在水溶液中与核苷PPs （K-app bbb10 (6) M-1）强结合，并通过双发射变化感应它们。详细的荧光和紫外/可见光谱研究表明，化学传感器与核苷PPs结合后的发射变化可归因于Zn-II与吖啶荧光团之间的配位丧失。这是一种独特的基于阴离子诱导的配位重排的传感系统。此外，我们证明了这些化学传感器在实时监测涉及核苷PP转化的两个重要生物过程中的实用性：apyase催化核苷PPs的水解和β -1,4-半乳糖转移酶催化的糖基转移。
Nucleoside pyrophosphate (nucleoside PP) derivatives are widespread in living cells and play pivotal roles in various biological events. We report novel fluorescence chemosensors for nucleoside PPs that make use of coordination chemistry. The chemosensors, which contain two Zn-II-dipicolylamine units, bind strongly to nucleoside PPs (K-app > 10(6) M-1) in aqueous solution and sense them by a dual-emission change. Detailed fluorescence and UV/Vis spectral studies revealed that the emission changes of the chemosensors upon binding to nucleoside PPs can be ascribed to the loss of coordination between Zn-II and the acridine fluorophore. This is a unique sensing system based on the anion-induced rearrangement of the coordination. Furthermore, we demonstrated the utility of these chemosensors in real-time monitoring of two important biological processes involving nucleoside PP conversion: the apyrase-catalyzed hydrolysis of nucleoside PPs and the glycosyl transfer catalyzed by beta-1,4-galactosyltransferase.