Adhesion to Vitronectin and Collagen I Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells.
Adhesion to Vitronectin and Collagen I Promotes Osteogenic Differentiation of Human Mesenchymal Stem Cells.
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DOI：
10.1155/s1110724304306017
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发表时间：
2004
影响因子：
--
通讯作者：
Plopper GE
中科院分区：
文献类型：
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作者：
Salasznyk RM;Williams WA;Boskey A;Batorsky A;Plopper GE
控制人间充质干细胞（hMSC）分化的机制尚不完全清楚。我们假设与通常在骨髓中发现的细胞外基质（ECM）蛋白接触会促进体外hMSC的成骨分化。为了验证这一假设，我们在存在或不存在可溶性成骨补充剂的情况下，在纯化的ECM蛋白上培养hMSC，并在16天的时间过程中检测已建立的分化标志物（矿化基质的产生、骨桥蛋白、骨钙素、胶原I和碱性磷酸酶的表达）的存在。我们发现hMSC通过不同的整合素受体以不同的亲和力粘附在ECM蛋白上（）。重要的是，最大的成骨分化发生在玻璃体连接蛋白和胶原I上，而在纤维连接蛋白或未包被的板上几乎没有分化。我们得出结论，与玻璃体粘连蛋白和I型胶原的接触促进了hMSC的成骨分化，并且仅与ECM接触可能足以诱导这些细胞的分化。
The mechanisms controlling human mesenchymal stem cells (hMSC) differentiation are not entirely understood. We hypothesized that the contact with extracellular matrix (ECM) proteins normally found in bone marrow would promote osteogenic differentiation of hMSC in vitro. To test this hypothesis, we cultured hMSC on purified ECM proteins in the presence or absence of soluble osteogenic supplements, and assayed for the presence of well-established differentiation markers (production of mineralized matrix, osteopontin, osteocalcin, collagen I, and alkaline phosphatase expression) over a 16-day time course. We found that hMSC adhere to ECM proteins with varying affinity () and through distinct integrin receptors. Importantly, the greatest osteogenic differentiation occurred in cells plated on vitronectin and collagen I and almost no differentiation took place on fibronectin or uncoated plates. We conclude that the contact with vitronectin and collagen I promotes the osteogenic differentiation of hMSC, and that ECM contact alone may be sufficient to induce differentiation in these cells.