Effects of recombinant tumor necrosis factor on proliferation and differentiation of leukemic and normal hemopoietic cells in vitro. Relationship to cell surface receptor.
Effects of recombinant tumor necrosis factor on proliferation and differentiation of leukemic and normal hemopoietic cells in vitro. Relationship to cell surface receptor.
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重组肿瘤坏死因子对白血病和正常造血细胞体外增殖和分化的影响。
DOI：
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发表时间：
1986
影响因子：
15.9
通讯作者：
I. Olsson
中科院分区：
文献类型：
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作者：
C. Peetre;U. Gullberg;E. Nilsson;I. Olsson
重组肿瘤坏死因子 (rTNF) 在 1-15 pM 浓度下可抑制骨髓性白血病细胞系的克隆生长。然而，野生型（早幼粒细胞）HL-60 细胞对生长抑制具有高度抵抗力，但在 100 pM rTNF 时会分化为单核细胞样细胞。新鲜急性髓系白血病细胞的克隆生长在约 15 pM rTNF 时被抑制 50%。正常粒细胞-巨噬细胞祖细胞 (CFU-GM) 的生长也受到抑制（50 pM rTNF），红系祖细胞 (BFU-E) 的生长（150 pM rTNF）也受到抑制。 rTNF 和重组干扰素-γ 之间具有协同抗增殖作用。使用放射性碘化 rTNF 使我们能够在 4°C 下检测骨髓细胞系上的 1,500-2,100 个结合位点，Kd 约为 300 pM。在 37 摄氏度下，结合配体转移至溶酶体后发生降解，并被 NH4+ 抑制。在 4°C 下的结合位点数量或亲和力与添加 rTNF 的抗增殖反应之间没有观察到相关性。
The clonogenic growth of myeloid leukemia cell lines was inhibited by recombinant tumor necrosis factor (rTNF) at 1-15 pM concentration. However, wild type (promyelocytic) HL-60 cells were highly resistant to growth inhibition, but responded with differentiation into monocyte-like cells at 100 pM rTNF. The clonogenic growth of fresh acute myeloid leukemia cells was inhibited by 50% at approximately 15 pM rTNF. The growth of normal granulocyte-macrophage progenitors (CFU-GM) was also inhibited (by 50 pM rTNF), as was the growth of erythroid progenitors (BFU-E) (by 150 pM rTNF). A synergistic antiproliferative effect was demonstrated between rTNF and recombinant interferon-gamma. Use of radioiodinated rTNF enabled us to detect 1,500-2,100 binding sites on myeloid cell lines at 4 degrees C with Kd of approximately 300 pM. At 37 degrees C, the transfer of bound ligand to lysosomes was followed by degradation, inhibited by NH4+. No correlation was observed between the number of binding sites or affinity at 4 degrees C and antiproliferative response to the addition of rTNF.