Development of Virus Concentration Method Using Negatively Charged Membrane by Alkaline Elution after Acid Rinse.
Development of Virus Concentration Method Using Negatively Charged Membrane by Alkaline Elution after Acid Rinse.
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开发使用带负电膜的病毒浓缩方法，通过酸洗后的碱洗脱。
DOI：
10.2965/jswe.25.469
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
S. Ohgaki
中科院分区：
文献类型：
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作者：
H. Katayama;Akihiro Shimasaki;S. Ohgaki
利用病毒、多价阳离子和膜之间的静电相互作用，建立了一种病毒浓缩方法。用带负电荷的膜吸附水样中的病毒，然后用稀硫酸（pH3.0-4.8）去除膜上的阳离子，再用碱性溶液（pH10.5 ~ 12）或牛肉膏溶液（pH9.5）洗脱病毒。以F特异性RNA噬菌体Qβ和脊髓灰质炎病毒I型为模型病毒，以纯净水、自来水和人工海水为样品。使用带负电荷的膜进行酸冲洗，然后进行碱洗脱的方法显示，在包括1 MDS带正电荷的膜在内的脊髓灰质炎病毒检测方法中，回收率最高，尤其是在应用于海水时。该方法还具有优于使用无机洗脱剂的常规方法的潜在优点，使得不会抑制随后通过PCR进行的病毒检测。
A virus concentration method was developed utilizing the electrostatic interaction among viruses, multivalent cations and membranes. A negatively charged membrane was used to adsorb viruses from water samples through which diluted sulfuric acid (pH3.0-4.8) was then passed to remove cations from the membrane, followed by the elution of viruses with alkaline solution (pH10.5-12) or with beef extract solution (pH9.5). F-specific RNA phage Qβ and poliovirus type I were used as model viruses and pure water, tap water and artificial seawater were used as samples. The method using a negatively charged membrane with acid rinse followed by alkaline elution showed the highest recovery yields among those tested for polioviruses including the 1MDS positively charged membrane, especially when applied to seawater. This method also has a potential advantage over conventional methods of using inorganic eluant such that there will be no inhibition of the subsequent virus detection by PCR.