MicroRNA expression profiles of peripheral blood mononuclear cells in patients with systemic lupus erythematosus
MicroRNA expression profiles of peripheral blood mononuclear cells in patients with systemic lupus erythematosus
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DOI：
10.1016/j.acthis.2014.02.009
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发表时间：
2014-01-01
影响因子：
2.5
通讯作者：
Wang, Xiaofei
中科院分区：
文献类型：
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作者：
Liu, Dongmei;Zhao, Haiyan;Wang, Xiaofei
系统性红斑狼疮(SLE)是一种自身免疫性疾病，以多种自身抗原自身抗体的存在为特征。有证据表明microRNAs(MiRNAs)在SLE发病机制中的重要性，但这种疾病的确切病因尚不清楚。因此，本研究利用基因芯片技术探讨异常miRNAs在系统性红斑狼疮中的调控作用。适当分离SLE患者和正常对照的外周血单个核细胞(PBMC)。提取每个细胞样本的总RNA，用于微阵列分析。共发现29个miRNAs在SLE患者PBMCs中表达下调(P<；0.05，Fold Change>；2)。在SLE患者中未发现明显上调的miRNAs。基因本体论分析结果表明，这些miRNAs的潜在靶基因主要集中在发育过程、转录调节活性、配体结合等方面，同时这些可能的基因还参与了多种信号转导途径，包括多条肿瘤通路、Wnt和丝裂原活化蛋白激酶信号通路等。本研究揭示了差异表达的miRNAs可能针对SLE的生物学过程和途径，提示这些miRNAs参与了SLE的发病机制。(C)2014年爱思唯尔股份有限公司。版权所有。
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the presence of autoantibodies against numerous self-antigens. Evidence underlines the importance of microRNAs (miRNAs) in the pathogenesis of SLE, but the exact etiology of this disease is unknown. Therefore, this study was conducted to explore regulation of abnormal miRNAs in SLE using microarray analysis. Peripheral blood mononuclear cells (PBMCs) from SLE patients and their matched controls were isolated appropriately. Total RNAs from each cell sample were extracted and used for microarray analysis. A total of 29 miRNAs were identified to be down-regulated in PBMCs of SLE patients as compared with healthy controls (P < 0.05, fold change > 2). No significant up-regulated miRNAs were found in SLE patients. Results of gene ontology analysis indicated that the potential target genes of these miRNAs mainly enriched in the development process, transcription regulator activity, ligand binding, etc. Meanwhile, these putative genes were also found to be involved in diverse signaling transduction pathways, including multiple cancer pathways, Wnt and mitogen-activated protein kinase signaling pathways, etc. This study revealed possible dysregulated biological processes and pathways in SLE that were targeted by the differentially expressed miRNAs, indicating the involvement of these miRNAs in the pathogenesis of SLE. (C) 2014 Elsevier GmbH. All rights reserved.