MicroRNA and 3T3-L1 pre-adipocyte differentiation
MicroRNA and 3T3-L1 pre-adipocyte differentiation
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DOI：
10.1261/rna.7228806
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发表时间：
2006-09-01
期刊：
影响因子：
4.5
通讯作者：
Iwai, Naoharu
中科院分区：
文献类型：
--
作者：
Kajimoto, Kazuaki;Naraba, Hiroaki;Iwai, Naoharu
microRNAs（miRNAs）在细胞增殖、凋亡和分化中发挥重要作用。在这项研究中，我们研究了3 T3-L1前脂肪细胞分化过程中的miRNA表达谱。我们从分化前后的3 T3-L1细胞中构建了miRNA文库，并鉴定了77个先前报道的miRNA和3个新的miRNA的表达。接下来，我们通过北方印迹分析研究了脂肪形成过程中102种miRNA的表达水平，包括文库中鉴定的那些。共检测到65个miRNAs，其中21个miRNAs在脂肪形成过程中表达上调或下调。有趣的是，在诱导分化后第9天观察到miRNA表达模式的变化，此时脂滴可见，但在第1、2或5天则没有。对上调的miRNA的反义抑制并不影响3 T3-L1前脂肪细胞分化。尽管这些miRNA可能参与调节脂肪细胞功能，但上调的miRNA的轻度下调似乎不影响3 T3-L1前脂肪细胞分化。
MicroRNAs ( miRNAs) have been suggested to play important roles in cell proliferation, apoptosis, and differentiation. In this study, we examined miRNA expression profiles during 3T3-L1 pre-adipocyte differentiation. We constructed miRNA libraries from pre- and post-differentiated 3T3-L1 cells, and identified the expression of 77 previously reported miRNAs and 3 new miRNAs. Next, we investigated the expression levels of 102 miRNAs, including those identified in the libraries, during adipogenesis by Northern blot analysis. Sixty-five miRNAs were detected and the expression of 21 miRNAs was up- or down-regulated during adipogenesis. Intriguingly, changes in the miRNA expression pattern were observed at day 9, when lipid droplets were visible, but not at days 1, 2, or 5 after the induction of differentiation. Antisense inhibition of the up- regulated miRNAs did not affect 3T3-L1 pre- adipocyte differentiation. Although these miRNAs may be involved in modulating adipocyte function, mild down-modulations of the up- regulated miRNAs do not appear to affect 3T3-L1 pre- adipocyte differentiation.