Biosynthesis of functionally active heparin cofactor II by a human hepatoma-derived cell line.
Biosynthesis of functionally active heparin cofactor II by a human hepatoma-derived cell line.
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人肝癌细胞系生物合成功能活性肝素辅因子 II。
DOI：
10.1016/0006-291x(85)91031-9
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发表时间：
1985
影响因子：
3.1
通讯作者：
Tollefsen,DM
中科院分区：
文献类型：
--
作者：
Jaffe,EA;Armellino,D;Tollefsen,DM
人血浆肝素辅因子II（HCII）通过在肝素或硫酸皮肤素存在下快速形成稳定的等摩尔复合物来抑制凝血酶。培养的人肝癌细胞（PLC PRF-5）分泌（3天内达到200 ng/ml）MW-72 kD的蛋白质，其经免疫分离并用抗HCII进行免疫印迹，在SDS-PAGE上与人血浆HCII共迁移，并与凝血酶形成共价复合物（MW-101 kD），存在但不存在肝素或硫酸皮肤素;这些复合物与通过在相同条件下将凝血酶与人血浆温育而获得的那些复合物共迁移。在培养的人脐静脉内皮细胞或人包皮成纤维细胞的培养后培养基中未检测到HCII（< 0.13 ng/ml
Human plasma heparin cofactor II (HCII) inhibits thrombin by rapidly forming a stable, equimolar complex in the presence of heparin or dermatan sulfate. Cultured human hepatoma-derived cells (PLC PRF-5) secreted (≈ 200 ng/ml in 3 days) a protein of MW-72 kD that was immunoisolated and immunoblotted with anti-HCII, co-migrated on SDS-PAGE with human plasma HCII, and formed covalent complexes with thrombin (MW-101 kD) in the presence but not absence of heparin or dermatan sulfate; these complexes co-migrated with those obtained by incubating thrombin with human plasma under the same conditions. HCII was not detectable (< 0.13 ng/ml) in post-culture medium from cultured human umbilical vein endothelial cells or human foreskin fibroblasts