Genes encoding putative Takeout/juvenile hormone binding proteins in the honeybee (Apis mellifera) and modulation by age and juvenile hormone of the takeout-like gene GB19811
Genes encoding putative Takeout/juvenile hormone binding proteins in the honeybee (Apis mellifera) and modulation by age and juvenile hormone of the takeout-like gene GB19811
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DOI：
10.1016/j.ibmb.2007.04.002
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发表时间：
2007-07-01
影响因子：
3.8
通讯作者：
Bloch, Guy
中科院分区：
文献类型：
--
作者：
Hagai, Tzachi;Cohen, Mira;Bloch, Guy
我们确定了8个基因编码假定的Takeout/保幼激素结合蛋白（To/JHBP）的蜜蜂基因组中的特征。系统发育分析揭示了这个基因家族中的9个不同谱系，包括那些含有Takeout（To）和JHBP的谱系，其中没有蜜蜂同源物。它们的多样性和无处不在的表达表明，To/JHBP蛋白在昆虫中参与了多种重要的过程。我们进一步表征了这些基因之一的表达，即广泛表达的GB 19811。GB 19811在腹部的转录水平随着工龄的增加而增加，在头部的转录水平随着工龄的增加而降低。蜂群环境和育雏行为对幼蜂中GB 19811的表达没有影响。用保幼激素（JH）处理的幼蜂显示头部GB 19811 mRNA水平下降。这一发现与JH的滴度通常随年龄增加而增加的前提一致，JH参与了GB 19811表达的年龄相关调节。与果蝇Takeout相反，GB 19811的表达不随昼夜节律或昼夜节律而变化。综上所述，这些发现表明GB 19811不是Takeout的直系同源物，并且参与JH介导的成年蜜蜂工蜂发育的调节。(c)2007爱思唯尔有限公司保留所有权利。
We identified and characterized eight genes encoding putative Takeout/juvenile hormone binding proteins (To/JHBP) in the honeybee genome. Phylogenetic analyses revealed nine distinct lineages within this gene family, including those containing Takeout (To) and JHBP for which there are no honeybee homologs. Their diversity and ubiquitous expression suggest that To/JHBP proteins are involved in diverse and important processes in insects. We further characterized the expression of one of these genes, GB19811 that is ubiquitously expressed. GB19811 transcript levels in the abdomen increased, and decreased in the head with worker age. There was no influence of colony environment or brood care behavior on GB19811 expression in young bees. Young bees treated with juvenile hormone (JH) showed a decrease in head GB19811 mRNA levels. This finding is consistent with the premise that JH, for which titers typically increase with age, is involved in age-related modulation of GB19811 expression. In contrast to Drosophila Takeout, the expression of GB19811 did not vary with diurnal or circadian rhythms. Taken together, these findings suggest that GB19811 is not an ortholog of Takeout, and is involved in JH-mediated regulation of adult honeybee worker development. (c) 2007 Elsevier Ltd. All rights reserved.