A monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues
A monoclonal antibody detects heterogeneity in vascular endothelium of tumours and normal tissues
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DOI：
10.1002/ijc.2910540303
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发表时间：
1993-05
影响因子：
6.4
通讯作者：
Ji Min Wang;Shant Kumar;D. Pye;A. Agthoven;J. Krupiński;R. Hunter
中科院分区：
文献类型：
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作者：
Ji Min Wang;Shant Kumar;D. Pye;A. Agthoven;J. Krupiński;R. Hunter
利用组织培养的人脐静脉内皮细胞制备了一种新的小鼠单克隆抗体（MAb） E - 9。该单抗识别的抗原在非还原条件下为170 kDa，在还原条件下为96 kDa。MAb E‐9在不同组织中的分布具有明显的异质性。它所识别的抗原存在于所有肿瘤的血管内皮细胞、胎儿器官以及再生和炎症组织中。它染色了一些正常组织。然而，除扁桃体外，泛内皮抗体（CD31）和血管性血变因子（另一种血管内皮标志物）抗体的双重染色显示，染色往往较弱，仅限于少数血管。令人惊讶的是，胎盘绒毛内的血管完全呈阴性。MAb E - 9识别抗原的功能尚不清楚，但其评估和使用应增加我们对血管生成的理解。
A new murine monoclonal antibody (MAb), E‐9, has been raised using tissue‐cultured human umbilical vein endothelial cells. The antigen recognized by this MAb is a peptide of 170 kDa under non‐reducing conditions and 96 kDa under reducing conditions. MAb E‐9 showed marked heterogeneity in its distribution in various tissues. The antigen recognized by it was present in vascular endothelial cells of all tumours, foetal organs and in regenerating and inflamed tissues. It stained a few normal tissues. However, with the exception of tonsils, staining tended to be weak and limited to a few blood vessels, as revealed by double staining using pan‐endothelial antibody (CD31) and antibody to von Willebrand factor, another marker of vascular endothelium. Surprisingly, blood vessels within the placental villi were completely negative. The function of the antigen recognized by MAb E‐9 is not known, but its evaluation and use should increase our understanding of angiogenesis.