Thick filament mechano-sensing is a calcium-independent regulatory mechanism in skeletal muscle.
Thick filament mechano-sensing is a calcium-independent regulatory mechanism in skeletal muscle.
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DOI：
10.1038/ncomms13281
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发表时间：
2016-10-31
影响因子：
16.6
通讯作者：
Irving, M.
中科院分区：
文献类型：
--
作者：
Fusi, L.;Brunello, E.;Yan, Z.;Irving, M.
最近对骨骼肌主动收缩纤维的x射线衍射研究表明，可与含有肌动蛋白的细丝相互作用的肌球蛋白马达的数量由含有肌动蛋白的粗丝中的应力控制。这些结果表明，粗丝机械传感可能是横纹肌中独立于众所周知的细丝介导的钙信号通路的一种新的调节机制。在这里，我们使用附着在脱膜肌纤维中肌球蛋白调节轻链的探针来检验这一假设。我们发现粗丝活化的程度和动力学都取决于粗丝应力，但在生理范围内与细胞内钙浓度无关。这些结果表明，通过粗丝机械传感直接控制肌球蛋白运动是骨骼肌中独立于典型钙信号通路的一般调节机制。最近的数据表明，除了众所周知的细丝介导的钙信号通路外，肌肉收缩还受粗丝机械传感的调节。在这里，作者提供了直接的证据，证明骨骼肌中的肌球蛋白激活是由粗丝应力控制的，独立于钙。
Recent X-ray diffraction studies on actively contracting fibres from skeletal muscle showed that the number of myosin motors available to interact with actin-containing thin filaments is controlled by the stress in the myosin-containing thick filaments. Those results suggested that thick filament mechano-sensing might constitute a novel regulatory mechanism in striated muscles that acts independently of the well-known thin filament-mediated calcium signalling pathway. Here we test that hypothesis using probes attached to the myosin regulatory light chain in demembranated muscle fibres. We show that both the extent and kinetics of thick filament activation depend on thick filament stress but are independent of intracellular calcium concentration in the physiological range. These results establish direct control of myosin motors by thick filament mechano-sensing as a general regulatory mechanism in skeletal muscle that is independent of the canonical calcium signalling pathway. Recent data suggest that muscle contraction is regulated by thick filament mechano-sensing in addition to the well-known thin filament-mediated calcium signalling pathway. Here the authors provide direct evidence that myosin activation in skeletal muscle is controlled by thick filament stress independently of calcium.
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影响因子：
5.6
作者：
HUXLEY, HE;BROWN, W
通讯作者：
BROWN, W
DOI：
10.1073/pnas.95.14.8052
发表时间：
1998-07-07
影响因子：
11.1
作者：
Linke, WA;Ivemeyer, M;Kolmerer, B
通讯作者：
Kolmerer, B
影响因子：
3.4
作者：
Linari, Marco;Caremani, Marco;Lombardi, Vincenzo
通讯作者：
Lombardi, Vincenzo
DOI：
10.1073/pnas.1602776113
发表时间：
2016-05-24
影响因子：
11.1
作者：
Kampourakis, Thomas;Sun, Yin-Biao;Irving, Malcolm
通讯作者：
Irving, Malcolm
影响因子：
5.5
作者：
Fusi, Luca;Brunello, Elisabetta;Lombardi, Vincenzo
通讯作者：
Lombardi, Vincenzo