Cell-to-Cell Spread of the RNA Interference Response Suppresses Semliki Forest Virus (SFV) Infection of Mosquito Cell Cultures and Cannot Be Antagonized by SFV
Cell-to-Cell Spread of the RNA Interference Response Suppresses Semliki Forest Virus (SFV) Infection of Mosquito Cell Cultures and Cannot Be Antagonized by SFV
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DOI：
10.1128/jvi.02440-08
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发表时间：
2009-06-01
影响因子：
5.4
通讯作者：
Kohl, Alain
中科院分区：
文献类型：
--
作者：
Attarzadeh-Yazdi, Ghassem;Fragkoudis, Rennos;Kohl, Alain
在它们的脊椎动物宿主中，虫媒病毒如塞姆利基森林病毒（SFV）（托加病毒科）通常会抵消先天防御并引发细胞死亡。相比之下，在蚊子细胞中，在早期有效的病毒产生阶段之后，建立了低水平病毒产生的持续感染。虫媒病毒是否能抵抗RNA干扰（RNAi）是一个重要的问题，RNAi为蚊子提供了一个重要的抗病毒防御系统。本研究表明，在白纹伊蚊衍生的蚊子细胞中，SFV不能阻止抗病毒RNAi应答的建立，也不能阻止保护性抗病毒双链RNA/小干扰RNA （siRNA）在细胞间的传播，而siRNA可以抑制传入病毒的复制。通过SFV表达的tombusvirus sirna结合蛋白p19强烈增强了病毒在培养细胞之间的传播，而不是病毒在初始感染细胞中的复制。我们的研究结果表明，RNAi信号的扩散有助于限制病毒的传播。
In their vertebrate hosts, arboviruses such as Semliki Forest virus (SFV) (Togaviridae) generally counteract innate defenses and trigger cell death. In contrast, in mosquito cells, following an early phase of efficient virus production, a persistent infection with low levels of virus production is established. Whether arboviruses counteract RNA interference (RNAi), which provides an important antiviral defense system in mosquitoes, is an important question. Here we show that in Aedes albopictus-derived mosquito cells, SFV cannot prevent the establishment of an antiviral RNAi response or prevent the spread of protective antiviral double-stranded RNA/small interfering RNA (siRNA) from cell to cell, which can inhibit the replication of incoming virus. The expression of tombusvirus siRNA-binding protein p19 by SFV strongly enhanced virus spread between cultured cells rather than virus replication in initially infected cells. Our results indicate that the spread of the RNAi signal contributes to limiting virus dissemination.