OXIDIZED LDL INDUCES ENHANCED ANTIBODY-FORMATION AND MHC CLASS II-DEPENDENT IFN-GAMMA PRODUCTION IN LYMPHOCYTES FROM HEALTHY-INDIVIDUALS
OXIDIZED LDL INDUCES ENHANCED ANTIBODY-FORMATION AND MHC CLASS II-DEPENDENT IFN-GAMMA PRODUCTION IN LYMPHOCYTES FROM HEALTHY-INDIVIDUALS
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DOI：
10.1161/01.atv.15.10.1577
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发表时间：
1995-10-01
影响因子：
8.7
通讯作者：
FROSTEGARD, J
中科院分区：
文献类型：
--
作者：
HUANG, YH;RONNELID, J;FROSTEGARD, J
动脉粥样硬化早期阶段的特点是 T 淋巴细胞和单核细胞均侵入动脉内膜。其中一些 T 淋巴细胞显示出激活迹象，但它们被激活的机制尚不清楚。单核细胞发育成巨噬细胞，随后发育成充满氧化低密度脂蛋白 (oxLDL) 衍生脂质的泡沫细胞。研究发现 OxLDL 具有多种促炎作用，包括增强内皮细胞和单核细胞的粘附性、单核细胞和 T 细胞的趋化性以及 T 细胞活化。酶联免疫斑点 (ELISPOT) 测定已被证明是检测分泌抗体或细胞因子的单细胞的灵敏方法。在这里，我们使用这种方法来表征体外暴露于 oxLDL 后 T 细胞细胞因子的分泌模式。在健康捐献者 (n=27) 的外周血单核细胞中，ELISPOT 检测到产生 INF-gamma 的细胞数量显着增加 (P
The early stages of atherosclerosis are characterized by penetration into the arterial intima by both T lymphocytes and monocytes. Some of these T lymphocytes show signs of activation, though the mechanisms by which they become activated are not known. The monocytes develop into macrophages and subsequently into foam cells filled with oxidized LDL (oxLDL)-derived lipids. OxLDL has been found to exert several proinflammatory effects, including enhanced adhesiveness of endothelial cells and monocytes, chemotaxis of monocytes and T cells, and T-cell activation. The enzyme-linked immunospot (ELISPOT) assay has been shown to be a sensitive method for detection of single cells secreting antibodies or cytokines. Here we have used this method to characterize the T-cell cytokine secretion pattern after exposure to oxLDL in vitro. In peripheral blood mononuclear cells from healthy donors (n=27), a significantly enhanced number of INF-gamma-producing cells was detected by ELISPOT (P