Endocytosis of simian virus 40 into the endoplasmic reticulum.
Endocytosis of simian virus 40 into the endoplasmic reticulum.
复制标题
邻胞病毒40进入内质网中的内吞作用。
DOI：
10.1083/jcb.109.6.2721
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发表时间：
1989-12
影响因子：
7.8
通讯作者：
Helenius, A
中科院分区：
文献类型：
--
作者：
Kartenbeck, J;Stukenbrok, H;Helenius, A
我们用生化和超微结构技术研究了SV-40对CV-1细胞的内吞作用。[35 S]甲硫氨酸放射性标记的SV-40与CV-1细胞结合的半衰期为25 min。大多数进入的病毒颗粒在数小时内未降解。电子显微镜显示，一些病毒通过包被囊泡进入内体/溶酶体途径，而大多数病毒通过小的未包被囊泡被内吞。在高多重性感染后，观察到总细胞相关病毒的三分之一进入ER，在病毒应用后1-2小时开始。病毒存在于作为ER延伸而产生的大的、管状的、光滑的膜网络中。结果描述了一种新的和独特的膜转运途径，允许内吞的病毒颗粒从质膜到ER的目标。
The endocytosis of SV-40 into CV-1 cells we studied using biochemical and ultrastructural techniques. The half-time of binding of [35S]methionine-radiolabeled SV-40 to CV-1 cells was 25 min. Most of the incoming virus particles remained undegraded for several hours. Electron microscopy showed that some virus entered the endosomal/lysosomal pathway via coated vesicles, while the majority were endocytosed via small uncoated vesicles. After infection at high multiplicity, one third of total cell-associated virus was observed to enter the ER, starting 1-2 h after virus application. The viruses were present in large, tubular, smooth membrane networks generated as extentions of the ER. The results describe a novel and unique membrane transport pathway that allows endocytosed viral particles to be targeted from the plasma membrane to the ER.