Structure of dengue virus: Implications for flavivirus organization, maturation, and fusion
Structure of dengue virus: Implications for flavivirus organization, maturation, and fusion
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DOI：
10.1016/s0092-8674(02)00660-8
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发表时间：
2002-03-08
期刊：
影响因子：
64.5
通讯作者：
Strauss, JH
中科院分区：
文献类型：
--
作者：
Kuhn, RJ;Zhang, W;Strauss, JH
通过冷冻电子显微镜技术，并将已知的糖蛋白E结构拟合到电子密度图中，黄病毒的首个结构已被确定。在脂质双分子层内的病毒核心，其结构的有序性不如由90个糖蛋白E二聚体组成的外部二十面体支架。每个二十面体不对称单位中的3个E单体不具有准等效对称环境。差值图显示了小膜蛋白M相对于覆盖其上的E二聚体支架的位置。该结构表明，黄病毒以及类似的甲病毒，采用一种融合机制，即糖蛋白E的结构域II的远端β桶插入细胞膜。
The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein E into the electron density map. The virus core, within a lipid bilayer, has a less-ordered structure than the external, icosahedral scaffold of 90 glycoprotein E dimers. The three E monomers per icosahedral asymmetric unit do not have quasiequivalent symmetric environments. Difference maps indicate the location of the small membrane protein M relative to the overlaying scaffold of E dimers. The structure suggests that flaviviruses, and by analogy also alphaviruses, employ a fusion mechanism in which the distal beta barrels of domain II of the glycoprotein E are inserted into the cellular membrane.