Fluorescence polarization: measurements with a photon-counting photometer.
Fluorescence polarization: measurements with a photon-counting photometer.
复制标题
荧光偏振：用光子计数光度计测量。
DOI：
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发表时间：
1978
影响因子：
1.6
通讯作者：
G. Mitchell
中科院分区：
文献类型：
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作者：
D. Jameson;G. Weber;R. Spencer;G. Mitchell
为了便于在低信噪比下进行精确的偏振测量，研制了一种T格式的光子计数偏振光度计。对仪器的光学和电子学进行了描述。给出了仪器性能的几个例子，包括在低激发分辨率下对皮摩尔荧光素溶液的测量和在5A分辨率下对吲哚(2×10(-4)M)的激发偏振光谱的测量。
A T-format, photon-counting polarization photometer was constructed to facilitate precise polarization measurements at low signal-to-noise ratios. The instrument optics and electronics are described. Several examples of the instrument's performance are given, including measurements on picomolar fluorescein solutions at low excitation resolution and the excitation polarization spectrum of indole (2 x 10(-4)M) at a resolution of 5 A.