The chondrocyte cell proliferation of a chitosan/silk fibroin/egg shell membrane hydrogels
The chondrocyte cell proliferation of a chitosan/silk fibroin/egg shell membrane hydrogels
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DOI：
10.1016/j.ijbiomac.2018.11.226
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发表时间：
2019-03-01
影响因子：
8.2
通讯作者：
Karimizarandi, Leila
中科院分区：
文献类型：
--
作者：
Adali, Terin;Kalkan, Rasime;Karimizarandi, Leila
关节软骨是一种细胞化不良、无血管化的结缔组织，可因创伤和骨关节炎而发生改变。组织工程策略涉及细胞，生物材料和生物活性剂的组合，特别是对受损关节软骨的修复感兴趣。本研究旨在设计壳聚糖/丝素/卵壳膜（CHI/SF/ESM）水凝胶，并分析其对人软骨细胞增殖活性的影响。采用FTIR分光光度计、XRD分析、ICP-MS、SEM分析、蛋白酶溶胀动力学和生物降解试验、大肠杆菌、白色念珠菌抑菌活性试验等方法对水凝胶进行表征。CHI/SF/ESM的水凝胶结构和ECM的化学均匀性都得到了很好的再现。将人关节软骨细胞接种于水凝胶上，在标准培养条件下培养2周。24 h、72 h和7 d时采用相差显微镜和MTT法观察软骨细胞的附着和细胞生长情况。在标准培养条件下，水凝胶支持软骨细胞更好的粘附、生长和分化。结果表明，CHI/SF/ESM水凝胶具有潜在的组织工程软骨替代品的应用前景。(C) 2018 Elsevier B.V.版权所有
Articular cartilage is a poorly cellularized, non-vascularized connective tissue that undergoes alterations due to trauma and osteoarthritis. Tissue engineering strategies involving the combination of cells, biomaterials and bioactive agents have been of interest notably for the repair of damaged articular cartilage. The aim of this study was to design Chitosan/Silk Fibroin/Egg Shell Membrane (CHI/SF/ESM) hydrogels and analyse the cell proliferation activity of human chondrocyte cells. The FTIR spectrophotometer, XRD analysis, ICP-MS, SEM analysis, swelling kinetics and biodegradation tests with protease enzyme, and antibacterial activity test with Escherichia coli, Candida albicans have been used for characterization of hydrogels. CHI/SF/ESM hydrogel structures, and chemical homogeneity of the ECM was well-reproduced. Human articular chondrocytes were seeded on hydrogels and cultured up to 2 weeks under the standard culture conditions. The attachment and cell growth of chondrocytes were examined by phase contrast microscopy and by MTT assay at 24 h, 72 h and 7 days. The hydrogel supported better adhesion, growth and differentiation of chondrocyte cells under standard culture conditions. The results obtained have suggested that, CHI/SF/ESM hydrogels can potentially function as a promising cartilage substitute for tissue engineering application. (C) 2018 Elsevier B.V. All rights reserved.