Quantitative determination of benzoylecgonine and cocaine in human biofluids by gas-liquid chromatography.
Quantitative determination of benzoylecgonine and cocaine in human biofluids by gas-liquid chromatography.
复制标题
采用气相色谱法定量测定人体生物体液中的苯甲酰爱康宁和可卡因。
DOI：
10.1021/ac50021a020
复制
发表时间：
1977
影响因子：
7.4
通讯作者：
S. Mulé
中科院分区：
文献类型：
--
作者：
M. Kogan;K. Verebey;A. DePace;R. Resnick;S. Mulé
本文用气相色谱法定量测定了可可碱（COC）及其在人体内的主要代谢产物苯甲酰芽子碱（BE）。从人血浆中提取后，COC采用氮检测，BE采用电子捕获检测。采用火焰离子化检测器同时测定人尿中的COC和BE。血浆中未衍生的COC和五氟苄基溴衍生物BE的检测限分别为10和5 ng/mL。尿中COC和BE的甲硅烷基衍生物的灵敏度分别为0.5和1.0 μ g/mL。变异系数在0. 9-2.2%，决定系数为0.99。从接受1.0至1.9 mg/kg可卡因HC 1的三名人类受试者在24小时内收集的COC和BE的血浆和尿液浓度数据。五是提出。
Cocaine (COC) and Its principal metabolite In man, benzoylecgonine (BE) were determined by quantitative gas-liquid chromatographic methods. Nitrogen detection was used for COC and electron capture detection for BE following extraction from human plasma. Using flame Ionization detection, COC and BE were simultaneously determined from human urine. The limits of detection for COC underivatlzed andBE as the pentafluorobenzyl bromide derivative In plasma were 10 and 5 ng/mL, respectively. In urine the sensitivity limits of the sllyl derivatives of COC andBE were 0.5 and 1.0 Mg/mL, respectively. The coefficient of variation ranged between 0. 9-2.2% and the coefficient of determination was 0.99 for these methods. Data on plasma and urine concentration of COC and BE collected over a time period of 24 h from three human subjects who received 1.0 to 1.9 mg/kg cocaine’HCI1. v. are presented.