Membrane redistribution of the Escherichia coli MinD protein induced by MinE.
Membrane redistribution of the Escherichia coli MinD protein induced by MinE.
复制标题
MinE 诱导的大肠杆菌 MinD 蛋白的膜重新分布。
DOI：
10.1128/jb.182.3.613-619.2000
复制
发表时间：
2000
影响因子：
3.2
通讯作者：
Rothfield,LI
中科院分区：
文献类型：
--
作者：
Rowland,SL;Fu,X;Sayed,MA;Zhang,Y;Cook,WR;Rothfield,LI
大肠埃希氏菌在中层细胞和与细胞极相邻的位置含有潜在的分裂位点。中细胞正确分裂部位的选择受三种蛋白质控制：Minc、Mind和My。以前已经证明(D.Raskin和P.de Boer，Cell 91：685-694,1997)，mine-GFP以一种精神依赖的方式定位于中细胞位置。我们在这里使用GFP-Mind是为了表明在没有其他Min蛋白的情况下，Mind与细胞整个外围的膜相关联，并且我的能够改变GFP-Mind的膜分布模式。对分离的N-末端和C-末端MINE结构域的研究表明，这两个MINE结构域在膜相关思维的重新分布中扮演着不同的角色。
Escherichia colicells contain potential division sites at midcell and adjacent to the cell poles. Selection of the correct division site at midcell is controlled by three proteins: MinC, MinD, and MinE. It has previously been shown (D. Raskin and P. de Boer, Cell 91:685–694, 1997) that MinE-Gfp localizes to the midcell site in an MinD-dependent manner. We use here Gfp-MinD to show that MinD associates with the membrane around the entire periphery of the cell in the absence of the other Min proteins and that MinE is capable of altering the membrane distribution pattern of Gfp-MinD. Studies with the isolated N-terminal and C-terminal MinE domains indicated different roles for the two MinE domains in the redistribution of membrane-associated MinD.