Observation of virus-like particles in high temperature enrichment cultures from deep-sea hydrothermal vents

Observation of virus-like particles in high temperature enrichment cultures from deep-sea hydrothermal vents
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DOI:
10.1016/s0923-2508(03)00075-5
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发表时间:
2003-05-01
影响因子:
2.6
通讯作者:
Prieur, D
Prieur, D
中科院分区:
生物学3区
文献类型:
--
作者:
Geslin, C;Le Romancer, M;Prieur, D

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A systematic search was carried out on samples collected in various geographically distant hydrothermal sites located on the East Pacific Rise (EPR 9degrees N and 13degrees N) and Mid-Atlantic Ridge (MAR 36degrees N and 37degrees N) to investigate the diversity of virus-like particles (VLPs) from deep-sea vents. Eighty-nine positive enrichment cultures were obtained from one hundred and one crude samples at 85 degreesC. VLPs were detected by electron microscopy in fifteen different enrichments. Among the different morphotypes observed, the lemon-shaped type prevailed but rods and novel pleomorphic morphologies were also observed. Several observations strongly suggested that host strains of the novel VLPs belong to the hyperthermophilic euryarchaeal order Thermococcales. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.