NOVEL HETEROTRIMERIC KINESIN-RELATED PROTEIN PURIFIED FROM SEA-URCHIN EGGS
NOVEL HETEROTRIMERIC KINESIN-RELATED PROTEIN PURIFIED FROM SEA-URCHIN EGGS
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DOI：
10.1038/366268a0
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发表时间：
1993-11-18
期刊：
影响因子：
64.8
通讯作者：
SCHOLEY, JM
中科院分区：
文献类型：
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作者：
COLE, DG;CHINN, SW;SCHOLEY, JM
运动蛋白重链和运动蛋白相关多肽(KRP)组成一个具有不同细胞内转运功能的运动蛋白家族1-7。使用与这些蛋白8，9反应的泛激肽抗体，我们先前已经从海胆卵中纯化了一种三聚体微管结合和捆绑蛋白Krp(85/95)(参考文献。8)由M(R)115,000(115K)、95K和85K亚基组成。我们在这里报道了与动蛋白相关的基因编码85K和95K亚基，并且该蛋白可以用85K单抗从胞浆中免疫沉淀为三聚体复合体。我们还发现，纯化的Krp(85/95)可引导运动向微管的“+”端移动。据我们所知，该蛋白是第一个从天然宿主细胞中以天然多聚体状态纯化的运动蛋白相关马达。
KINESIN heavy chain and kinesin-related polypeptides (KRPs) comprise a family of motor proteins with diverse intracellular transport functions1-7. Using pan-kinesin peptide antibodies that react with these proteins8,9, we have previously purified from sea urchin eggs a trimeric microtubule-binding and bundling protein, KRP(85/95) (ref. 8) comprising subunits of M(r) 115,000 (115K), 95K and 85K. We report here that kinesin-related genes encode the 85K and 95K subunits, and that the protein can be immunoprecipitated from cytosol as a trimeric complex using an 85K monoclonal antibody. We also find that purified KRP(85/95) directs movements towards the 'plus' ends of microtubules. To our knowledge, this protein is the first kinesin-related motor to be purified from its natural host cell in a native multimeric state.