Effect of two GABA-ergic drugs on the cognitive functions of rapid eye movement in sleep-deprived and recovered rats
Effect of two GABA-ergic drugs on the cognitive functions of rapid eye movement in sleep-deprived and recovered rats
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DOI：
10.3892/etm.2016.3445
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发表时间：
2016-08-01
影响因子：
2.7
通讯作者：
Bo, Agula
中科院分区：
文献类型：
--
作者：
Bao, Lidao;Si, Lengge;Bo, Agula
快速眼动（REM）睡眠与神经功能密切相关。本研究旨在评价加巴嗪和噻加宾对REM睡眠剥夺和睡眠恢复大鼠认知功能的影响。将大鼠分为REM睡眠剥夺组、空白对照组和环境组。REM睡眠剥夺组又分为非手术组（nonOP）、假手术组（Sham）、加巴嗪组（gabazine，SR）和噻加宾组（tiagabine，SR）。各组在5个时间点进行评估：睡眠剥夺1d（SD 1d）、SD 3d、SD 5d、睡眠恢复6h（RS 6h）和RS 12h。采用改良的多平台水法建立大鼠REM睡眠剥夺模型，Morris水迷宫检测CF。下丘脑。-通过高效液相色谱法测定氨基丁酸（GABA）和谷氨酸含量。免疫荧光法检测下丘脑泌素（Hcrt）神经元和Fos的数量、形态及GABA（A）R α 1诱导的积分光密度。与CC组相比，nonOP组和Sham组大鼠CF明显减少，Fos阳性细胞和Fos-Hcrt双阳性细胞明显增多，GABA含量和GABA（A）R α 1表达水平明显升高（P
Rapid eye movement (REM) sleep is closely associated with nervous functions. The present study aimed to evaluate the effects of gabazine and tiagabine on the cognitive functions (CF) of REM sleep-deprived and sleep recovered rats. Rats were divided into REM sleep deprivation, blank control (CC) and environmental groups. The REM sleep deprivation group was further divided into non-operation (nonOP), sham-operated (Sham), gabazine (SR) and tiagabine groups. Each group was evaluated over five time points: Sleep deprived for 1 day (SD 1 day), SD 3 day, SD 5 day, sleep recovery 6 h (RS 6 h) and RS 12 h. A rat model of REM sleep deprivation was established by a modified multi-platform water method, with CF assessed by Morris water maze. Hypothalamic.-aminobutyric acid (GABA) and glutamic acid contents were measured via high performance liquid chromatography. The number and morphology of hypocretin (Hcrt) neurons and Fos in the hypothalamus, and GABA(A)R alpha 1-induced integral optical density were detected by immunofluorescence. Compared to the CC group, the nonOP and Sham group rats CF were significantly diminished, Fos-positive and Fos-Hcrt double positive cells were significantly increased, and GABA content and GABA(A)R alpha 1 expression levels were significantly elevated (P