Characterization of the T cell receptor repertoire in the Japanese neuromyelitis optica: T cell activity is up-regulated compared to multiple sclerosis
Characterization of the T cell receptor repertoire in the Japanese neuromyelitis optica: T cell activity is up-regulated compared to multiple sclerosis
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DOI：
10.1016/j.jns.2006.06.011
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发表时间：
2006-11-15
影响因子：
4.4
通讯作者：
Matsumoto, Yoh
中科院分区：
文献类型：
--
作者：
Warabi, Yoko;Yagi, Kohichi;Matsumoto, Yoh
为了表征日本视神经肌萎缩症（NMO）患者的T细胞免疫，我们采用互补决定区3（CDR3）光谱法检测了NMO患者的T细胞受体（TCR）库，并将结果与多发性硬化症（MS）患者和健康受试者的结果进行了比较。与健康受试者相比，NMO和MS患者都有更多克隆扩增的V β基因。此外，NMO患者的扩展VP数量明显多于MS患者。详细的分析表明，V I和V β 13显着激活NMO比MS。这些结果反映了独特的病理生理学的日本NMO，这是从MS的区别。此外，纵向检查的TCR剧目表明，在NMO的克隆扩展V β的数量与Kurtzke扩展残疾状态量表（EDSS）。尽管复发缓解型MS（RRMS）中TCR库的激活模式与NMO相似，但病程较长且EDSS评分较高的继发性进展型MS（SPMS）患者的克隆性扩增V β的数量始终低于RRMS患者。详细的TCR研究将为评估NMO和MS的临床和免疫状态以及开发有效的免疫治疗提供有用的信息。(c)2006 Elsevier B.V.保留所有权利。
To characterize T cell immunity in Japanese neuromyelitis optica (NMO), we examined the T cell receptor (TCR) repertoire in NMO patients with complementarity-determining region 3 (CDR3) spectratyping and compared the results with those from multiple sclerosis (MS) patients and healthy subjects. Both NMO and MS patients had a larger number of clonally expanded V beta genes than healthy subjects. Moreover, NMO patients had a significantly larger number of expanded VPs than MS patients. The detailed analysis revealed that V I and V beta 13 were significantly activated in NMO than MS. These results reflected unique pathophysiology of Japanese NMO, which is distinguishable from that of MS. Furthermore, longitudinal examinations of the TCR repertoire demonstrated that the number of clonally expanded V beta s in NMO correlates with the Kurtzke Expanded disability status scale (EDSS). Although the activation pattern of the TCR repertoire in relapsing-remitting MS (RRMS) was similar to that in NMO, secondary progressive MS (SPMS) patients with longer disease durations and higher EDSS scores consistently had a smaller number of clonally expanded V beta s than RRMS patients. Detailed TCR investigations will provide useful information to evaluate the clinical and immunological status of NMO and MS and to develop effective immunotherapies. (c) 2006 Elsevier B.V. All rights reserved.