Solid-phase synthesis of human pancreatic polypeptide.
Solid-phase synthesis of human pancreatic polypeptide.
复制标题
人胰多肽的固相合成。
DOI：
10.1111/j.1399-3011.1980.tb02584.x
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
D. Coy
中科院分区：
文献类型：
--
作者：
C. Meyers;D. Coy
采用固相法合成人胰多肽（HPP）的36个残基序列，经HF处理从二苯甲胺树脂上裂解，凝胶过滤、离子交换和分配层析纯化。除常规标准外，还通过反相高效液相色谱法检查了最终产品的均匀性。该技术还用于分离和表征溴化氰切割片段，并最终将合成产物与从天然来源分离的天然人、牛和猪胰多肽进行比较。纯化的HPP与天然PP一样，是犬胰腺分泌的有效抑制剂。
The 36-residue sequence of human pancreatic polypeptide (HPP) was synthesized by solid-phase methodology, cleaved from the benzhydrylamine resin by HF treatment, and purified by gel filtration, ion exchange, and partition chromatography. In addition to the usual criteria, the homogeneity of the final product was examined by reversed phase high performance liquid chromatography. This technique was also used to isolate and characterize the cyanogen bromide cleavage fragments, and finally to compare the synthetic product with native human, bovine, and porcine pancreatic polypeptide isolated from natural sources. The purified HPP, like native PP, was a potent inhibitor of pancreatic secretion in the dog.