Axin, an inhibitor of the Wnt signalling pathway, interacts with β-catenin, GSK-3β and APC and reduces the β-catenin level
Axin, an inhibitor of the Wnt signalling pathway, interacts with β-catenin, GSK-3β and APC and reduces the β-catenin level
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DOI：
10.1046/j.1365-2443.1998.00198.x
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发表时间：
1998-06-01
期刊：
影响因子：
2.1
通讯作者：
Akiyama, T
中科院分区：
文献类型：
--
作者：
Nakamura, T;Hamada, F;Akiyama, T
背景：Wnt/Wingless信号通路在胚胎发育和肿瘤发生中起重要作用。结果：我们发现Axin与β-catenin和糖原合成酶激酶-3 β（GSK-3 β）相互作用。此外，Axin的G蛋白信号传导（RGS）结构域的调节与结直肠肿瘤抑制因子腺瘤性结肠息肉病（APC）相关。Axin在人结肠直肠癌细胞系SW 480中的过表达诱导β-连环蛋白水平的急剧降低。结论：Axin与β-catenin、GSK-3 β和APC相互作用，负性调节Wnt信号通路，推测其可能是通过调节β-catenin的水平。
Background: The Wnt/Wingless signalling pathway plays an important role in both embryonic development and tumorigenesis. beta-Catenin and Axin are positive and negative effectors of the Wnt signalling pathway, respectively.Results: We found that Axin interacts with beta-catenin and glycogen synthase kinase-3 beta (GSK-3 beta). Furthermore, the regulation of the G-protein signalling (RGS) domain of Axin is associated with the colorectal tumour suppressor adenomatous polyposis coli (APC). Overexpression of Axin in the human colorectal cancer cell line SW480 induced a drastic reduction in the level of beta-catenin. Interaction with beta-catenin and GSK-3 beta was required for the Axin-mediated beta-catenin reduction.Conclusion: Axin interacts with beta-catenin, GSK-3 beta and APC, and negatively regulates the Wnt signalling pathway, presumably by regulating the level of beta-catenin.