Effect of intraluminal pillars on particle motion in bifurcated microchannels.
Effect of intraluminal pillars on particle motion in bifurcated microchannels.
复制标题
腔内柱对分叉微通道中粒子运动的影响。
DOI：
10.1007/s11626-008-9134-9
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
Mentzer,StevenJ
中科院分区：
文献类型：
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作者：
Turhan,Aslihan;Tsuda,Akira;Konerding,MoritzA;Lin,Miao;Miele,Lino;Lee,Grace;Mentzer,StevenJ
肠套叠血管生成的一个中心特征是血管内支柱的形成，该支柱将微血管管腔的相对两侧连接起来。在这份报告中，我们根据结肠微循环的腐蚀铸型创建了具有几何比例的聚二甲基硅氧烷(PDMS)微通道。PDMS微通道的结构是一个分叉的通道，在分叉的几何中心有一个管腔内的柱子。使用体外灌流系统研究了管腔内柱子对颗粒流动路径的影响。微通道内注入荧光颗粒，用荧光视频显微镜记录颗粒运动。我们发现，血管内柱子的存在显著降低了分叉系统中的粒子速度(p<； )。此外，立柱还改变了气流中心线上颗粒的运动轨迹。颗粒轨迹导致柱子接触时间延长，以及在分叉系统中的停留时间增加(p<； 0.001)。我们的结果表明，血管内支柱不仅提供了一种增加血流阻力的机制，还可能参与了血流中细胞的空间重新分布。
A central feature of intussusceptive angiogenesis is the development of an intravascular pillar that bridges the opposing sides of the microvessel lumen. In this report, we created polydimethyl siloxane (PDMS) microchannels with geometric proportions based on corrosion casts of the colon microcirculation. The structure of the PDMS microchannels was a bifurcated channel with an intraluminal pillar in the geometric center of the bifurcation. The effect of the intraluminal pillar on particle flow paths was investigated using an in vitro perfusion system. The microchannels were perfused with fluorescent particles, and the particle movements were recorded using fluorescence videomicroscopy. We found that the presence of an intravascular pillar significantly decreased particle velocity in the bifurcation system (p< 0.05). In addition, the pillar altered the trajectory of particles in the center line of the flow stream. The particle trajectory resulted in prolonged pillar contact as well as increased residence time within the bifurcation system (p< 0.001). Our results suggest that the intravascular pillar not only provides a mechanism of increasing resistance to blood flow but may also participate in spatial redistribution of cells within the flow stream.