Idiosyncratic neural coding and neuromodulation of olfactory individuality in Drosophila
Idiosyncratic neural coding and neuromodulation of olfactory individuality in Drosophila
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DOI：
10.1073/pnas.1901623116
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发表时间：
2020-09-22
影响因子：
11.1
通讯作者：
de Bivort, Benjamin L.
中科院分区：
文献类型：
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作者：
Honegger, Kyle S.;Smith, Matthew A-Y;de Bivort, Benjamin L.
相同基因的个体之间先天的行为偏向和偏好可能会有很大的不同。虽然个性是行为的基本属性，但目前还不清楚大脑结构和生理上的个体差异是如何产生特殊行为的。在这里，我们提出了果蝇嗅觉行为和神经反应的特殊性的证据。我们发现，来自高度近亲繁殖的实验室品系的个体雌性果蝇表现出持续数天的特殊气味偏好。我们使用神经反应的活体钙成像来比较投射神经元(将气味信息从感觉外周传递到中央大脑的二阶神经元)在不同动物中对相同气味的反应。我们发现，虽然气味反应看起来非常刻板，但仔细观察，天线上的许多个体差异都很明显！小叶(AL)肾小球(对应于不同气味通道的紧凑微回路)。此外，我们还表明，神经调节、以营养改变形式的环境应激以及某些AL局部中间神经元的活动影响苍蝇间行为变异性的大小。综上所述，这项工作表明，个体果蝇表现出特殊的嗅觉偏好和特殊的神经对气味的反应，行为特殊受到AL中神经元的神经调节和调节。
Innate behavioral biases and preferences can vary significantly among individuals of the same genotype. Though individuality is a fundamental property of behavior, it is not currently understood how individual differences in brain structure and physiology produce idiosyncratic behaviors. Here we present evidence for idiosyncrasy in olfactory behavior and neural responses in Drosophila. We show that individual female Drosophila from a highly inbred laboratory strain exhibit idiosyncratic odor preferences that persist for days. We used in vivo calcium imaging of neural responses to compare projection neuron (second-order neurons that convey odor information from the sensory periphery to the central brain) responses to the same odors across animals. We found that, while odor responses appear grossly stereotyped, upon closer inspection, many individual differences are apparent across antenna! lobe (AL) glomeruli (compact microcircuits corresponding to different odor channels). Moreover, we show that neuromodulation, environmental stress in the form of altered nutrition, and activity of certain AL local interneurons affect the magnitude of interfly behavioral variability. Taken together, this work demonstrates that individual Drosophila exhibit idiosyncratic olfactory preferences and idiosyncratic neural responses to odors, and that behavioral idiosyncrasies are subject to neuromodulation and regulation by neurons in the AL.