Mutagenesis Analysis of GMN Motif in Arabidopsis thaliana Mg2+ Transporter MRS2-1

Mutagenesis Analysis of GMN Motif in Arabidopsis thaliana Mg2+ Transporter MRS2-1
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拟南芥Mg2转运蛋白MRS2-1 GMN基序的诱变分析

DOI:
10.1093/bbb/zbac064
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发表时间:
2022
期刊:
Bioscience, Biotechnology, and Biochemistry
影响因子:
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通讯作者:
Tanoi Keitaro
Tanoi Keitaro
中科院分区:
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文献类型:
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作者:
Yang Xiaoyu;Kobayashi Natsuko I;Hayashi Yoshiki;Ito Koichi;Moriwaki Yoshitaka;Terada Tohru;Shimizu Kentaro;Hattori Motoyuki;Iwata Ren;Suzuki Hisashi;Nakanishi Tomoko M;Tanoi Keitaro

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镁是植物的重要营养元素,但植物镁离子转运蛋白的研究还很不清楚。结合AlphaFold 2的结构预测,我们利用突变和28 Mg摄取实验对拟南芥MRS 2 -1(AtMRS 2 -1)转运蛋白的高度保守的“GMN”基序进行了研究。我们证明了GMN基序中的甘氨酸和蛋氨酸是AtMRS 2 -1转运Mg 2+所必需的。
Magnesium is an important nutrient for plants, but much is still unknown about plant Mg2+transporters. Combining with the structural prediction of AlphaFold2, we used mutagenesis and28Mg uptake assay to study the highly conserved “GMN” motif ofArabidopsis thalianaMRS2-1 (AtMRS2-1) transporter. We demonstrated that the glycine and methionine in GMN motif are essential for AtMRS2-1 to transport Mg2+.