Infectious cDNA clones of four viroids in Coleus blumei and molecular characterization of their progeny
Infectious cDNA clones of four viroids in Coleus blumei and molecular characterization of their progeny
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彩叶草中四种类病毒的感染性 cDNA 克隆及其后代的分子特征
DOI：
10.1016/j.virusres.2013.11.014
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发表时间：
2014-02-13
期刊：
影响因子：
5
通讯作者：
Li, Shifang
中科院分区：
文献类型：
--
作者：
Jiang, Dongmei;Gao, Rui;Li, Shifang
报道了4种侵染彩叶草的类病毒，命名为彩叶草类病毒1-4（CbVd-1-CbVd-4），以及2个拟新种。命名为CbVd-5和CbVd-6，已对其进行了表征，其中两种（CbVd-5和CbVd-6，2009年首次报道）尚未建立生物测定法。在这里，感染性克隆被用作接种源，并评估CbVd-1、-3、-5和-6的生物学特性。当对体外合成的二聚体CbVd（+）RNA进行生物测定时，四种CbVd的首次检测时间不同，从45天到300天不等。此外，我们证实了CbVd-5和CbVd-6可以通过种子传播。接种后一年，从斜线接种植物的后代的分子特征表明，CbVd群体的遗传多样性可能取决于受感染的彩叶草植物和类病毒基因型。(C)2013爱思唯尔有限公司版权所有。
Four viroid species infecting Coleus blumei, named Coleus blumei viroid 1-4 (CbVd-1-CbVd-4), and two tentative new viroid species,. named CbVd-5 and CbVd-6, have been characterized, for two of which (CbVd-5 and CbVd-6, first reported in 2009), there is no established bioassay. Here, infectious clones were used as inoculums sources and the biological properties of CbVd-1, -3, -5 and -6 were assessed. When dimeric CbVd (+) RNAs synthesized in vitro were bioassayed, the first detection time for the four CbVds was different, ranging from 45 to 300 days. In addition, we confirmed that CbVd-5 and CbVd-6 can be transmitted via seeds. Molecular characterization of their progeny from slash-inoculated plants one year after inoculation demonstrated that the genetic diversity of CbVd populations may depend on the infected coleus plants and on the viroid genotype. (C) 2013 Elsevier B.V. All rights reserved.