Binding sites for inositol trisphosphate in the bovine adrenal cortex.
Binding sites for inositol trisphosphate in the bovine adrenal cortex.
复制标题
牛肾上腺皮质中肌醇三磷酸的结合位点。
DOI：
10.1016/0006-291x(85)90939-8
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Catt,KJ
中科院分区：
文献类型：
--
作者：
Baukal,AJ;Guillemette,G;Rubin,R;Spät,A;Catt,KJ
用[32 P] ATP放射性标记人红细胞制备[32 P] IP 3，在牛肾上腺皮质中鉴定了三磷酸肌醇（IP 3）的结合位点。IP 3以高亲和力（Kd= 5 nM）和低容量（186 fmol/mg蛋白）与肾上腺微粒体结合。在动力学研究期间，在4° C下在不到1分钟内达到半最大结合，并且解离甚至更快，t12为约10秒。[32 IP 2未显示与微粒体位点结合，微粒体位点代表IP 3在肽激素如血管紧张素II作用期间升高细胞内钙浓度的假定受体。
Binding sites for inositol trisphosphate (IP 3) have been identified in bovine adrenal cortex, employing [32 P] IP 3 prepared from human erythrocytes radiolabeled with [32 P] ATP. IP 3 was bound to adrenal microsomes with high affinity (Kd= 5 nM) and low capacity (186 fmol/mg protein). During kinetic studies, half-maximal binding was reached in less than one min at 4° C, and dissociation was even more rapid with t 1 2 of about 10 sec.[32 P] IP 2 showed no binding to the microsomal sites, which represent putative receptors at which IP 3 acts to elevate intracellular calcium concentration during the actions of peptide hormones such as angiotensin II.