Increased immunoreactivity to SLIT/ROBO1 in ovarian endometriomas: a likely constituent biomarker for recurrence.
Increased immunoreactivity to SLIT/ROBO1 in ovarian endometriomas: a likely constituent biomarker for recurrence.
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DOI：
10.2353/ajpath.2009.090024
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发表时间：
2009-08
期刊：
影响因子：
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通讯作者：
Fanghua Shen;Xi-shi Liu;J. Geng;Sun-Wei Guo
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文献类型：
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作者：
Fanghua Shen;Xi-shi Liu;J. Geng;Sun-Wei Guo
虽然手术是目前治疗子宫内膜异位症的选择，复发仍然是一个严重的问题，其预防是一个未满足的临床需求。SLIT是一种分泌性蛋白，通过Roundabout（ROBO）受体作为轴突导向和神经元迁移的排斥剂发挥作用，最近发现它可诱导肿瘤血管生成。我们研究了SLIT/ROBO 1在卵巢腺瘤中的潜在作用，并基于43例复发患者和45例未复发患者的组织样本检测了其复发预测价值。通过CD 34免疫组化评估微血管密度计数，并进行统计分析，以评估SLIT/Robo 1对调整其他风险因素后复发风险的影响。我们发现SLIT的表达与微血管密度呈正相关，并且其在异位内膜中的表达高于对照内膜。SLIT和Robo 1在复发病例中的表达均高于非复发病例。对SLIT的高免疫反应性，沿着粘连、PR-B和核因子-κ B的存在，被确定为复发的危险因素，敏感性为86%，特异性为87%。因此，SLIT免疫反应性增加可能是卵巢恶性肿瘤复发的一个重要因素，可能通过促进异位内膜血管生成。因此，SLIT/ROBO 1系统可能是降低复发风险的潜在靶点。
While surgery is currently the treatment of choice for endometriosis, recurrence remains a serious problem, and its prevention is an unmet clinical need. SLIT, a secreted protein that functions through the Roundabout (ROBO) receptor as a repellent for axon guidance and neuronal migration, has been recently found to induce tumor angiogenesis. We investigated the potential role of SLIT/ROBO1 in ovarian endometriomas and examined their predictive value in recurrence based on tissue samples from 43 patients with recurrence and 45 without recurrence. Microvascular density counts were evaluated by CD34 immunohistochemistry, and statistical analyses were performed to evaluate the effect of SLIT/Robo1 on recurrence risk after adjustment for other risk factors. We found that SLIT expression was positively correlated with microvascular density in ectopic endometrium and that its expression was higher in ectopic endometrium than control endometrium. Both SLIT and Robo1 expression were higher in recurrent cases than in non-recurrent cases. Higher immunoreactivity to SLIT, along with the presence of adhesion, PR-B, and nuclear factor-kappaB, was identified to be a risk factor for recurrence, with a sensitivity of 86% and a specificity of 87%. Therefore, increased SLIT immunoreactivity is likely an important constituent factor for recurrence of ovarian endometriomas, possibly through promoting angiogenesis in ectopic endometrium. Thus, the SLIT/ROBO1 system may be a potential target for reducing the risk of recurrence.