Olfactory Enrichment Influences Adult Neurogenesis Modulating GAD67 and Plasticity-Related Molecules Expression in Newborn Cells of the Olfactory Bulb
Olfactory Enrichment Influences Adult Neurogenesis Modulating GAD67 and Plasticity-Related Molecules Expression in Newborn Cells of the Olfactory Bulb
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DOI：
10.1371/journal.pone.0006359
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发表时间：
2009-07-23
期刊：
影响因子：
3.7
通讯作者：
De Marchis, Silvia
中科院分区：
文献类型：
--
作者：
Bovetti, Serena;Veyrac, Alexandra;De Marchis, Silvia
嗅球（OB）是成年哺乳动物大脑的高度可塑性区域，其特征是颗粒（GC）和球周细胞（PGC）类型的抑制性中间神经元的持续整合。成人产生的 OB 中间神经元被选择以依赖于经验的方式生存，但介导经验对 OB 神经发生影响的机制尚不清楚。这里我们重点关注由多个亚型组成的新生PGC群体。使用嗅觉富集和/或剥夺的范例与 BrdU 注射和定量共聚焦免疫组织化学分析相结合，我们研究了嗅觉体验对不同存活时间的成体产生的 PGC 的影响，并将 PGC 与 GC 调制进行比较。我们发现，嗅觉丰富同样会影响 PGC 和 GC，从而增加新生细胞的存活率并瞬时调节 GAD67 和可塑性相关分子的表达。然而，与 GC 相比，PGC 的成熟似乎被延迟，反映了成人产生的嗅觉中间神经元整合的不同时间动态。此外，嗅觉丰富或剥夺不会选择性地调节特定 PGC 表型的存活，这支持了不同 PGC 亚型的整合率独立于嗅觉体验的观点。
The olfactory bulb (OB) is a highly plastic region of the adult mammalian brain characterized by continuous integration of inhibitory interneurons of the granule (GC) and periglomerular cell (PGC) types. Adult-generated OB interneurons are selected to survive in an experience-dependent way but the mechanisms that mediate the effects of experience on OB neurogenesis are unknown. Here we focus on the new-generated PGC population which is composed by multiple subtypes. Using paradigms of olfactory enrichment and/or deprivation combined to BrdU injections and quantitative confocal immunohistochemical analyses, we studied the effects of olfactory experience on adult-generated PGCs at different survival time and compared PGC to GC modulation. We show that olfactory enrichment similarly influences PGCs and GCs, increasing survival of newborn cells and transiently modulating GAD67 and plasticity-related molecules expression. However, PGC maturation appears to be delayed compared to GCs, reflecting a different temporal dynamic of adult generated olfactory interneuron integration. Moreover, olfactory enrichment or deprivation do not selectively modulate the survival of specific PGC phenotypes, supporting the idea that the integration rate of distinct PGC subtypes is independent from olfactory experience.