A role for T cell-derived interleukin 22 in psoriatic skin inflammation
A role for T cell-derived interleukin 22 in psoriatic skin inflammation
复制标题
DOI：
10.1111/j.1365-2249.2007.03511.x
复制
发表时间：
2007-12-01
影响因子：
4.6
通讯作者：
Morel, F.
中科院分区：
文献类型：
--
作者：
Boniface, K.;Guignouard, E.;Morel, F.
白细胞介素 (IL)-22 是一种 T 细胞衍生的细胞因子，最近有报道称其可在实验性银屑病小鼠模型中诱导皮肤炎症，并在体外诱导炎症样表型。在本研究中，我们评估了皮损、皮肤源性 T 细胞中 IL-22 和 IL-22 受体 1 (IL-22R1) 的存在情况，以及牛皮癣患者血清中 IL-22 的水平。 IL-22R1 和 IL-10R2 转录物在银屑病皮肤和健康皮肤中的表达水平相似。相反，与正常皮肤相比，银屑病皮损中 IL-22 mRNA 表达上调，而银屑病患者和正常受试者外周血单个核细胞中 IL-22 mRNA 水平相似。银屑病患者的循环 IL-22 水平显着高于正常受试者。与从同一患者中分离的外周 T 细胞相比，从银屑病皮肤中分离的 T 细胞产生更高水平的 IL-22。银屑病患者和正常受试者的皮肤活检和外周血单核细胞中 IL-10 的表达水平相似。最后，我们在此表明，皮损银屑病皮肤浸润 T 细胞的上清液可诱导正常人表皮角质形成细胞的炎症反应，类似于在银屑病皮损中观察到的炎症反应。总而言之，本研究报告的结果表明，IL-22 是一种由皮肤浸润淋巴细胞产生的细胞因子，可能参与银屑病发病机制的启动和/或维持。
Interleukin (IL)-22 is a T cell-derived cytokine that has been reported recently to induce cutaneous inflammation in an experimental murine model of psoriasis, and to induce in vitro an inflammatory-like phenotype. In the present study, we assessed the presence of IL-22 and the IL-22 receptor 1 (IL-22R1) in skin lesions, skin-derived T cells, as well as IL-22 levels in sera from patients with psoriasis. IL-22R1 and IL-10R2 transcripts are expressed at a similar level in psoriatic and healthy skin. In contrast, IL-22 mRNA expression was up-regulated in psoriatic skin lesions compared to normal skin, whereas IL-22 mRNA levels in peripheral blood mononuclear cells from psoriatic patients and normal subjects were similar. Circulating IL-22 levels were significantly higher in psoriatic patients than in normal subjects. T cells isolated from psoriatic skin produced higher levels of IL-22 in comparison to peripheral T cells isolated from the same patients. IL-10 was expressed at similar levels in skin biopsies and peripheral blood mononuclear cells of psoriatic patients and normal subjects. Finally, we show here that supernatants of lesional psoriatic skin-infiltrating T cells induce an inflammatory response by normal human epidermal keratinocytes, resembling that observed in psoriatic lesions. Taken together, the results reported in this study indicate that IL-22 is a cytokine produced by skin-infiltrating lymphocytes that is potentially involved in initiation and/or maintenance of the pathogenesis of psoriasis.