Decreased expression of human class II antigens on monocytes from patients with acquired immune deficiency syndrome. Increased expression with interferon-gamma.
Decreased expression of human class II antigens on monocytes from patients with acquired immune deficiency syndrome. Increased expression with interferon-gamma.
复制标题
获得性免疫缺陷综合征患者的单核细胞上人类 II 类抗原的表达减少。
DOI：
10.1172/jci111633
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Finberg,R
中科院分区：
文献类型：
--
作者：
Heagy,W;Kelley,VE;Strom,TB;Mayer,K;Shapiro,HM;Mandel,R;Finberg,R
应用双重荧光染色和细胞荧光定量法，对正常人和获得性免疫缺陷综合征（AIDS）患者外周血单核细胞HLA-DR（一种Ⅱ类组织相容性抗原）的表达进行了研究。在动物模型中，II类阳性单核细胞的缺乏与T细胞对抗原应答的失败有关。我们现在报道艾滋病患者缺乏HLA-DR+单核细胞。8名正常人中HLA-DR+单核细胞的百分比范围为49.3%至95.0%+，只有1名个体的HLA-DR+单核细胞低于50%。男性同性恋淋巴结病患者单核细胞HLA-DR表达与正常人相似（范围，58.0 ~ 97.4%+）。相比之下，9名艾滋病患者中有7名的HLA-DR+单核细胞低于50%（范围为13.4%至78.8%+）。来自AIDS患者的单核细胞与克隆的人干扰素-γ的体外孵育导致HLA-DR表达增加至接近正常水平。
The expression of HLA-DR (a class II histocompatibility antigen) on monocytes isolated from the peripheral blood of normal individuals and patients with acquired immune deficiency syndrome (AIDS) was investigated by the use of dual fluorescent staining and cytofluorometry. In animal models the absence of class II positive monocytes is linked to a failure of T cells to respond to antigens. We now report that patients with AIDS have a paucity of HLA-DR+ monocytes. The percentage of HLA-DR+ monocytes among eight normal individuals ranged from 49.3 to 95.0%+, and only one individual had less than 50% HLA-DR+ monocytes. HLA-DR expression on monocytes from homosexual male patients with lymphadenopathy was similar to that of normal subjects (range, 58.0 to 97.4%+). In contrast, seven of nine patients with AIDS had less than 50% HLA-DR+ monocytes (range, 13.4 to 78.8%+). The in vitro incubation of monocytes from AIDS patients with cloned human interferon-gamma resulted in an increase of the expression of HLA-DR to near normal levels.